Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods  

作　　者：Rokhaya Gueye Viviane Chatchueng Sandefo Babacar Beye Elhadji Ousmane Faye Amadou Diop Serigne Omar Sarr Bara Ndiaye Yérim Mbagnick Diop Rokhaya Gueye;Viviane Chatchueng Sandefo;Babacar Beye;Elhadji Ousmane Faye;Amadou Diop;Serigne Omar Sarr;Bara Ndiaye;Yérim Mbagnick Diop(Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal;Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal)

机构地区：[1]Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal [2]Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal

出　　处：《Food and Nutrition Sciences》2022年第11期950-961,共12页食品与营养科学（英文）

摘　　要：Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.

关 键 词：Poultry Feed AFLATOXIN High Performance Liquid Chromatography Thin-Layer Chromatography DAKAR 

分 类 号：F42[经济管理—产业经济]