Protection of Allergic Asthma in Mice by Black Rice Bran Bioprocessed with Shiitake Mushroom Mycelia  

作　　者：Ki Sun Kwon Woon Sang Hwang Kyung Hee Lee Kyung Jin Kim Wha Young Lee Jeanman Kim Sang Jong Lee Sung Phil Kim Mendel Friedman Ki Sun Kwon;Woon Sang Hwang;Kyung Hee Lee;Kyung Jin Kim;Wha Young Lee;Jeanman Kim;Sang Jong Lee;Sung Phil Kim;Mendel Friedman(STR Biotech Co., Ltd., Chuncheon, Republic of Korea;U.S. Department of Agriculture, Western Regional Research Center, Agricultural Research Services, Albany, CA, USA)

机构地区：[1]STR Biotech Co., Ltd., Chuncheon, Republic of Korea [2]U.S. Department of Agriculture, Western Regional Research Center, Agricultural Research Services, Albany, CA, USA

出　　处：《Food and Nutrition Sciences》2023年第4期341-368,共28页食品与营养科学（英文）

摘　　要：We evaluated the potential of orally fed new food formulations to inhibit biomarkers reported to be involved in the causes of allergic asthma in mice. Asthma, a serious non-communicable disease, affects both adults and children and can be undertreated. New functional foods could provide therapeutic approaches. Here, the anti-asthma mechanism of a new functional food and three isolated fractions produced by bioprocessing black rice bran with shiitake mushroom mycelia was evaluated in mast cells, B cells, and orally fed mice and compared with non-bioprocessed black rice bran. In vitro, the treatments inhibited RBL-2H3 cell degranulation and immunoglobulin E (IgE) production. The in vitro anti-asthma effects were confirmed in orally fed mice following asthma induction by alumina and chicken egg ovalbumin (OVA). The suppression of asthma resulted from the inhibition of inflammation- and immune-related substances, including OVA-specific IgE, thymic stromal lymphopoietin, eotaxin, leukotriene C4, prostaglandin D2, and vascular cell adhesion molecule-1 in bronchoalveolar lavage fluid and serum. The treatment also reversed the thickening of the lung airway wall. The inflammation and asthma inhibition seems to be regulated by the balance of the T-helper cells’ Th1/Th2 immune response and the inhibition of multiple biomarkers associated with the cause of asthma. Future human clinical studies with adults and children should determine the potential therapeutic value of the anti-asthma effects of the new functional foods.We evaluated the potential of orally fed new food formulations to inhibit biomarkers reported to be involved in the causes of allergic asthma in mice. Asthma, a serious non-communicable disease, affects both adults and children and can be undertreated. New functional foods could provide therapeutic approaches. Here, the anti-asthma mechanism of a new functional food and three isolated fractions produced by bioprocessing black rice bran with shiitake mushroom mycelia was evaluated in mast cells, B cells, and orally fed mice and compared with non-bioprocessed black rice bran. In vitro, the treatments inhibited RBL-2H3 cell degranulation and immunoglobulin E (IgE) production. The in vitro anti-asthma effects were confirmed in orally fed mice following asthma induction by alumina and chicken egg ovalbumin (OVA). The suppression of asthma resulted from the inhibition of inflammation- and immune-related substances, including OVA-specific IgE, thymic stromal lymphopoietin, eotaxin, leukotriene C4, prostaglandin D2, and vascular cell adhesion molecule-1 in bronchoalveolar lavage fluid and serum. The treatment also reversed the thickening of the lung airway wall. The inflammation and asthma inhibition seems to be regulated by the balance of the T-helper cells’ Th1/Th2 immune response and the inhibition of multiple biomarkers associated with the cause of asthma. Future human clinical studies with adults and children should determine the potential therapeutic value of the anti-asthma effects of the new functional foods.

关 键 词：Black Rice Bran Shiitake Mushrooms Bioprocessed Functional Food Mast Cells Mice Feeding Studies BIOASSAYS Cytokines IMMUNOGLOBULINS Bronchial Lavage Fluid Histology Anti-Inflammatory Effect Asthma Prevention Research Needs 

分 类 号：R56[医药卫生—呼吸系统]