<i>In Vitro</i>Susceptibility of Dermatophytes to Anti-Fungal Drugs and Aqueous <i>Acacia nilotica</i>Leaf Extract in Lagos, Nigeria  

作　　者：Muinah A. Fowora Faustina U. Onyeaghasiri Abdul Lateef O. Olanlege Idowu O. Edu-Muyideen Olumide O. Adebesin Muinah A. Fowora;Faustina U. Onyeaghasiri;Abdul Lateef O. Olanlege;Idowu O. Edu-Muyideen;Olumide O. Adebesin(Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria;FOWM Biotechnology Limited, Lagos, Nigeria;Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria;Department of Microbiology, Nigerian Institute of Medical Research, Lagos, Nigeria;Department of Cell Biology and Genetics, University of Lagos, Lagos, Nigeria)

机构地区：[1]Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria [2]FOWM Biotechnology Limited, Lagos, Nigeria [3]Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria [4]Department of Microbiology, Nigerian Institute of Medical Research, Lagos, Nigeria [5]Department of Cell Biology and Genetics, University of Lagos, Lagos, Nigeria

出　　处：《Journal of Biomedical Science and Engineering》2021年第2期74-82,共9页生物医学工程（英文）

摘　　要：Dermatophytes were earlier reported to respond well to anti-fungal agents;however, an upsurge in resistance with the high cost of these agents increased the use of medicinal plants for treatment. This study investigated the sensitivity pattern of dermatophytes to oral anti-fungal drugs and aqueous leaf extract of the plant, <em>Acacia nilotica</em>. The extract was tested against seven strains of dermatophytes <em>Arthroderma otae</em>, <em>Trichophyton interdigitale</em>, <em>Trichophyton mentagrophyte</em>, <em>Microsporum ferrugineum</em>, <em>Arthroderma vespertilii</em>, <em>Arthroderma quadrifidum</em>, and <em>Arthroderma multifidum</em>, previously isolated from diabetic patients. The minimum inhibitory and fungicidal concentrations of the plant extracts and the standard antifungal agents were evaluated using modifications of the broth macro dilution method of the National Committee for Clinical Laboratory Standards M38-A2 protocol. There was a significant difference in the Minimum Inhibitory concentrations (MIC) of the dermatophytes to the three antifungal drugs tested (p < 0.001). The dermatophytes were mostly susceptible to itraconazole followed by Nystatin. All the dermatophytes tested were resistant to griseofulvin. <em>Acacia nilotica</em> had an inhibitory effect on all the dermatophytes tested, and showed anti-fungal activity in a dose-dependent relationship between 0.625 - 1.25 mg/ml. Though the inhibitions of the dermatophytes were significantly higher with the standard anti-fungal drugs as compared to the plant extract (p < 0.001);however, the dermatophyte, <em>Arthroderma quadrifidum</em>, which was resistant to all the anti-fungal drugs, had the highest inhibition with <em>A. nilotica</em>. Some circulating dermatophyte strains in Nigeria are griseofulvin and/or itraconazole resistant which may influence the spread of infection and <em>A. nilotica</em> aqueous leaf extract showed a strong anti-dermatophytic activity.Dermatophytes were earlier reported to respond well to anti-fungal agents;however, an upsurge in resistance with the high cost of these agents increased the use of medicinal plants for treatment. This study investigated the sensitivity pattern of dermatophytes to oral anti-fungal drugs and aqueous leaf extract of the plant, <em>Acacia nilotica</em>. The extract was tested against seven strains of dermatophytes <em>Arthroderma otae</em>, <em>Trichophyton interdigitale</em>, <em>Trichophyton mentagrophyte</em>, <em>Microsporum ferrugineum</em>, <em>Arthroderma vespertilii</em>, <em>Arthroderma quadrifidum</em>, and <em>Arthroderma multifidum</em>, previously isolated from diabetic patients. The minimum inhibitory and fungicidal concentrations of the plant extracts and the standard antifungal agents were evaluated using modifications of the broth macro dilution method of the National Committee for Clinical Laboratory Standards M38-A2 protocol. There was a significant difference in the Minimum Inhibitory concentrations (MIC) of the dermatophytes to the three antifungal drugs tested (p < 0.001). The dermatophytes were mostly susceptible to itraconazole followed by Nystatin. All the dermatophytes tested were resistant to griseofulvin. <em>Acacia nilotica</em> had an inhibitory effect on all the dermatophytes tested, and showed anti-fungal activity in a dose-dependent relationship between 0.625 - 1.25 mg/ml. Though the inhibitions of the dermatophytes were significantly higher with the standard anti-fungal drugs as compared to the plant extract (p < 0.001);however, the dermatophyte, <em>Arthroderma quadrifidum</em>, which was resistant to all the anti-fungal drugs, had the highest inhibition with <em>A. nilotica</em>. Some circulating dermatophyte strains in Nigeria are griseofulvin and/or itraconazole resistant which may influence the spread of infection and <em>A. nilotica</em> aqueous leaf extract showed a strong anti-dermatophytic activity.

关 键 词：Acacia nilotica Anti-Fungal Agent DERMATOPHYTES Minimum Inhibitory Concentration Minimum Fungicidal Concentration 

分 类 号：R28[医药卫生—中药学]