Global Proteomics of the Extremophile Black Fungus <i>Cryomyces antarcticus</i>Using 2D-Electrophoresis  

作　　者：Kristina Zakharova Katja Sterflinger Ebrahim Razzazi-Fazeli Katharina Noebauer Gorji Marzban 

机构地区：[1]Austrian Center of Biological Resources and Applied Mycology, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria [2]Plant Biotechnology Unit, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria [3]Proteomics Unit, VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria

出　　处：《Natural Science》2014年第12期978-995,共18页自然科学期刊（英文）

摘　　要：The microcolonial black fungus Cryomyces antarcticus is an extremophile organism growing on and in rock in the Antarctic desert. Ecological plasticity and stress tolerance make it a perfect model organism for astrobiology. 2D-gel electrophoresis and MALDI-TOF/TOF mass spectrometry were performed to explore the protein repertoire, which allows the fungus to survive in the harsh environment. Only a limited number of proteins could be identified by using sequence homologies in public databases. Due to the rather low identification rate by sequence homology, this study reveals that a major part of the proteome of C. antarcticus varies significantly from other fungal species.The microcolonial black fungus Cryomyces antarcticus is an extremophile organism growing on and in rock in the Antarctic desert. Ecological plasticity and stress tolerance make it a perfect model organism for astrobiology. 2D-gel electrophoresis and MALDI-TOF/TOF mass spectrometry were performed to explore the protein repertoire, which allows the fungus to survive in the harsh environment. Only a limited number of proteins could be identified by using sequence homologies in public databases. Due to the rather low identification rate by sequence homology, this study reveals that a major part of the proteome of C. antarcticus varies significantly from other fungal species.

关 键 词：Microcolonial Fungi EXTREMOPHILES PROTEOMICS Protein Characterization 

分 类 号：R73[医药卫生—肿瘤]