Investigation on HTLV Infection among Voluntary Blood Donors in Wuzhou City  

作　　者：Lifei Liang Haiyan Wang Minzhen Wu Lifei Liang;Haiyan Wang;Minzhen Wu(Wuzhou Central Blood Station Laboratory, Wuzhou, China)

机构地区：[1]Wuzhou Central Blood Station Laboratory, Wuzhou, China

出　　处：《Natural Science》2022年第8期322-327,共6页自然科学期刊（英文）

摘　　要：Objective: To understand the infection of HTLV among voluntary blood donors in Wuzhou City, and to provide reference for the national health administrative department to formulate blood safety screening strategies. Methods: The HTLV double-antigen sandwich ELISA reagent was used to screen the blood samples of unpaid blood donors, and the reactive samples in the initial screening were subjected to a double-well retest;Specimens that were still reactive in the retest were further confirmed by viral nucleic acid amplification test (PCR) and western blotting (WB). Results: A total of 9 of 20,222 unpaid blood donation samples were screened to be reactive, and the screening response rate was 0.04%;Two samples of HTLV-1 nucleic acid and western blotting (WB) were confirmed to be positive, and the other seven samples were negative;The confirmed positive rate was 0.01%. Conclusion: There was a certain positive rate of HTLV-1 serological screening among the non remunerated blood donors in Wuzhou City, and the confirmation test confirmed that there was a certain risk of HTLV infection;In order to further understand the HTLV infection of blood donors in this city, we should further increase the number of screening samples, so as to obtain more reliable and accurate data in this region, and provide data and reference for the health administration department to formulate HTLV screening strategies for blood donors.Objective: To understand the infection of HTLV among voluntary blood donors in Wuzhou City, and to provide reference for the national health administrative department to formulate blood safety screening strategies. Methods: The HTLV double-antigen sandwich ELISA reagent was used to screen the blood samples of unpaid blood donors, and the reactive samples in the initial screening were subjected to a double-well retest;Specimens that were still reactive in the retest were further confirmed by viral nucleic acid amplification test (PCR) and western blotting (WB). Results: A total of 9 of 20,222 unpaid blood donation samples were screened to be reactive, and the screening response rate was 0.04%;Two samples of HTLV-1 nucleic acid and western blotting (WB) were confirmed to be positive, and the other seven samples were negative;The confirmed positive rate was 0.01%. Conclusion: There was a certain positive rate of HTLV-1 serological screening among the non remunerated blood donors in Wuzhou City, and the confirmation test confirmed that there was a certain risk of HTLV infection;In order to further understand the HTLV infection of blood donors in this city, we should further increase the number of screening samples, so as to obtain more reliable and accurate data in this region, and provide data and reference for the health administration department to formulate HTLV screening strategies for blood donors.

关 键 词：Unpaid Blood Donors HTLV-I/II Positive Rate Western Blotting 

分 类 号：R44[医药卫生—诊断学]