Expression of B Locus Beta 2 (<i>BLB2</i>) Gene at Cytolytic and Latent Immune Response Stages of Immunocompetence in Nigerian Indigenous Chickens  

作　　者：Abdulraheem Adedeji Bello-Ibiyemi Adeyemi Sunday Adenaike Micheal Irewole Takeet Akpan Ubong Christian Obiora Ndubuisi Ikeobi Abdulraheem Adedeji Bello-Ibiyemi;Adeyemi Sunday Adenaike;Micheal Irewole Takeet;Akpan Ubong;Christian Obiora Ndubuisi Ikeobi(Department of Animal Breeding and Genetics, College of Animal Science and Livestock Production, Federal University of Agriculture Abeokuta, Ogun State, Nigeria.;Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Ogun State, Nigeria.)

机构地区：[1]Department of Animal Breeding and Genetics, College of Animal Science and Livestock Production, Federal University of Agriculture Abeokuta, Ogun State, Nigeria. [2]Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Ogun State, Nigeria.

出　　处：《Open Journal of Animal Sciences》2021年第2期164-174,共11页动物科学期刊（英文）

摘　　要：An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"></span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">^–ΔΔCт<span style="white-space:nowrap;"></span>) wAn economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"></span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">^–ΔΔCт<span style="white-space:nowrap;"></span>) w

关 键 词：Nigerian Indigenous Chickens BLB2 Gene Expression Sheep Red Blood Cells 

分 类 号：O17[理学—数学]