Assessment of the Quality of Frozen-Thawed Semen or Epididymal Sperm in Three Native Vietnamese Pig Breeds  

作　　者：Van Khanh Nguyen Huong Thu Thi Vu Huong Le Thi Nguyen Huong Thi Nguyen Au Thi Hoang Dat Van Le Nhung Tuyet Thi Nguyen Hieu Trung Phan Yen Kim Thi Pham Son Phan Le Quynh Anh Le Quynh Anh Le Lan Doan Pham Minh Quang Luu Muoi Thi Nguyen Van Khanh Nguyen;Huong Thu Thi Vu;Huong Le Thi Nguyen;Huong Thi Nguyen;Au Thi Hoang;Dat Van Le;Nhung Tuyet Thi Nguyen;Hieu Trung Phan;Yen Kim Thi Pham;Son Phan Le;Quynh Anh Le;Quynh Anh Le;Lan Doan Pham;Minh Quang Luu;Muoi Thi Nguyen(Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Science, Hanoi, Vietnam;National Institute of Animal Science, Hanoi, Vietnam;Ministry of Science and Technology, Hanoi, Vietnam;Animal Experiments and Domestic Animal Conservation Center, National Institute of Animal Science, Hanoi, Vietnam)

机构地区：[1]Key Laboratory of Animal Cell Biotechnology, National Institute of Animal Science, Hanoi, Vietnam [2]National Institute of Animal Science, Hanoi, Vietnam [3]Ministry of Science and Technology, Hanoi, Vietnam [4]Animal Experiments and Domestic Animal Conservation Center, National Institute of Animal Science, Hanoi, Vietnam

出　　处：《Open Journal of Animal Sciences》2023年第3期283-297,共15页动物科学期刊（英文）

摘　　要：Our aim was to evaluate the quality of ejaculated and epididymal frozen-thawed pig sperm of endangered Vietnam native pig breeds. Ejaculated sperm was collected from live boars and epididymal sperm was collected from slaughtered boars of the MuongTe, Kieng Sat and Co BinhThuan breeds and frozen in 0.25 ml straws using a protocol established earlier for modern pig breeds. We evaluated the sperm quality after thawing in terms of motility and rates of viable and abnormal spermatozoa. Our results revealed that the sperm motility and rates of viable and abnormal frozen-thawed sperm were >30%, >44%, and <14%, respectively. The origin of sperm had an effect on the production of pig embryos in vitro. In the Co BinhThuan breed, ejaculated sperm generated higher cleavage, blastocyst and hatching rates than did the epididymal sperm (60.11% vs 56.02%, 17.23% vs 14.31%, 3.78% vs 2.34%, respectively, P < 0.05). Although no difference in cleavage rate, blastocyst formation rate and the average number of cells/blastocysts, the hatching blastocyst rate was different between the breeds (P > 0.05). In the Co BinhThuan breed, the rate of pregnancy of ejaculated groups was similar to that of the epididymal group. In conclusion, the ejaculated and epididymal sperm of native Vietnamese pigs were successfully frozen. We succeeded in creating embryos in vitro and pregnant pigs after artificial insemination from frozen-thawed semen in three native Vietnamese pig breeds for the first time. The use of the ejaculated sperm improved the production of native pig embryos in vitro efficiency.Our aim was to evaluate the quality of ejaculated and epididymal frozen-thawed pig sperm of endangered Vietnam native pig breeds. Ejaculated sperm was collected from live boars and epididymal sperm was collected from slaughtered boars of the MuongTe, Kieng Sat and Co BinhThuan breeds and frozen in 0.25 ml straws using a protocol established earlier for modern pig breeds. We evaluated the sperm quality after thawing in terms of motility and rates of viable and abnormal spermatozoa. Our results revealed that the sperm motility and rates of viable and abnormal frozen-thawed sperm were >30%, >44%, and <14%, respectively. The origin of sperm had an effect on the production of pig embryos in vitro. In the Co BinhThuan breed, ejaculated sperm generated higher cleavage, blastocyst and hatching rates than did the epididymal sperm (60.11% vs 56.02%, 17.23% vs 14.31%, 3.78% vs 2.34%, respectively, P < 0.05). Although no difference in cleavage rate, blastocyst formation rate and the average number of cells/blastocysts, the hatching blastocyst rate was different between the breeds (P > 0.05). In the Co BinhThuan breed, the rate of pregnancy of ejaculated groups was similar to that of the epididymal group. In conclusion, the ejaculated and epididymal sperm of native Vietnamese pigs were successfully frozen. We succeeded in creating embryos in vitro and pregnant pigs after artificial insemination from frozen-thawed semen in three native Vietnamese pig breeds for the first time. The use of the ejaculated sperm improved the production of native pig embryos in vitro efficiency.

关 键 词：Native Vietnamese Pig In Vitro Porcine Embryo Epididymal EJACULATE Artificial Insemination 

分 类 号：S82[农业科学—畜牧学]