Development of an Enteric Bacterial Enrichment Broth and Its Performance for Isolation of Clinically Significant Bacterial Pathogens from Stool  

作　　者：Timothy S. Horseman Michael B. Lustik Keith S. K. Fong Timothy S. Horseman;Michael B. Lustik;Keith S. K. Fong(Department of Clinical Investigation, Tripler Army Medical Center, Honolulu, USA)

机构地区：[1]Department of Clinical Investigation, Tripler Army Medical Center, Honolulu, USA

出　　处：《Open Journal of Medical Microbiology》2021年第1期1-17,共17页医学微生物学（英文）

摘　　要：<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:Verdana;"> Early detection and accurate identification of foodborne pathogen outbreaks is an important public health function. Increased clinical adoption of multiplex PCR assays or culture</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">independent diagnostic tests (CIDT) correlates to more stool specimens</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">sent to public health laboratories (PHL) for characterization. Isolation and confirmation of enteric bacterial </span><span style="font-family:Verdana;">pathogens can prove difficult to consistently recover. The purpose of this study</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">was to evaluate </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">performance of a broad-use laboratory developed enrichment </span><span><span style="font-family:Verdana;">broth for isolation of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Shigella</span></i><span style="font-family:Verdana;">, and </span><i><span style="font-family:Verdana;">Yersinia</span></i> </span><span style="font-family:Verdana;">strains from stool specimens. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> The study compared differences in positivity rates among media and enrichment combinations at specific time </span><span style="font-family:Verdana;">points. Comparison of direct inoculation (DI)</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> enrichment using a</span><span style="font-family:Verdana;"> lab-developed E<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:Verdana;"> Early detection and accurate identification of foodborne pathogen outbreaks is an important public health function. Increased clinical adoption of multiplex PCR assays or culture</span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">independent diagnostic tests (CIDT) correlates to more stool specimens</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">sent to public health laboratories (PHL) for characterization. Isolation and confirmation of enteric bacterial </span><span style="font-family:Verdana;">pathogens can prove difficult to consistently recover. The purpose of this study</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">was to evaluate </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">performance of a broad-use laboratory developed enrichment </span><span><span style="font-family:Verdana;">broth for isolation of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Salmonella</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Shigella</span></i><span style="font-family:Verdana;">, and </span><i><span style="font-family:Verdana;">Yersinia</span></i> </span><span style="font-family:Verdana;">strains from stool specimens. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> The study compared differences in positivity rates among media and enrichment combinations at specific time </span><span style="font-family:Verdana;">points. Comparison of direct inoculation (DI)</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> enrichment using a</span><span style="font-family:Verdana;"> lab-developed E

关 键 词：Enteric Bacteria ENRICHMENT STOOL Selective Agar 

分 类 号：O17[理学—数学]