Antifungal Susceptibility Testing of Dermatophytes by ABDD and E-Test, a Comparative Study  

作　　者：Matheus Heita Namidi Tejashree Ananthnaraja Badveti Satyasai Matheus Heita Namidi;Tejashree Ananthnaraja;Badveti Satyasai(Applied Microbiology, Parasitology and Mycology, Oshakati Campus University of Namibia, Oshakati, Namibia;Department of Microbiology, J.S.S Medical College and Hospital, Mysuru, India)

机构地区：[1]Applied Microbiology, Parasitology and Mycology, Oshakati Campus University of Namibia, Oshakati, Namibia [2]Department of Microbiology, J.S.S Medical College and Hospital, Mysuru, India

出　　处：《Open Journal of Medical Microbiology》2021年第3期129-143,共15页医学微生物学（英文）

摘　　要：<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span <b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span

关 键 词：DERMATOPHYTOSIS Dermatophyte Test Medium Disc Diffusion and E-Strips Antifungal Agents 

分 类 号：O17[理学—数学]