<i>In Vitro</i>Evaluation of Bacterial Adhesion to Dental and Stainless-Steel Surfaces  

作　　者：A. El Aouame F. El Quars Z. Bentahar K. Zerouali M. Sidqui A. El Aouame;F. El Quars;Z. Bentahar;K. Zerouali;M. Sidqui(Department of Orthodontics and Dentofacial Orthopedics, Faculty of Dentistry, Hassan II University, Casablanca, Morocco;Department of Microbiology, Faculty of Medicine and Pharmacology, Hassan II University, Casablanca, Morocco;Department of Biochemical, Faculty of Dentistry, Hassan II University, Casablanca, Morocco)

机构地区：[1]Department of Orthodontics and Dentofacial Orthopedics, Faculty of Dentistry, Hassan II University, Casablanca, Morocco [2]Department of Microbiology, Faculty of Medicine and Pharmacology, Hassan II University, Casablanca, Morocco [3]Department of Biochemical, Faculty of Dentistry, Hassan II University, Casablanca, Morocco

出　　处：《Open Journal of Medical Microbiology》2021年第3期176-197,共22页医学微生物学（英文）

摘　　要：<span style="font-family:Verdana;"></span><span style="font-family:Verdana;">This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainless-steel surfaces.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">We studied </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> the behavior of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti</span></i><span style="font-family:Verdana;">s in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120<span style="white-space:nowrap;">&#730;</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti S</span></i><span style="font-family:Verdana;"> on dental fragments was compared to </span><span style="font-family:Verdana;">the one obtained on stainless steel fragments. We also carried a Bacterial</span><span style="font-family:Verdana;"> count by optical dosing.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mai<span style="font-family:Verdana;"></span><span style="font-family:Verdana;">This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainless-steel surfaces.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">We studied </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> the behavior of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti</span></i><span style="font-family:Verdana;">s in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120<span style="white-space:nowrap;">&#730;</span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of </span><i><span style="font-family:Verdana;">Staphylococcus aureus Méti S</span></i><span style="font-family:Verdana;"> on dental fragments was compared to </span><span style="font-family:Verdana;">the one obtained on stainless steel fragments. We also carried a Bacterial</span><span style="font-family:Verdana;"> count by optical dosing.</span></span><span style="font-family:;" "=""> </span><span style="font-family:Verdana;">The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mai

关 键 词：Bacterial Adhesion Mechanism Staphylococcus aureus Méti Tooth Surface Stainless Steel 

分 类 号：TG1[金属学及工艺—金属学]