Phenotypic Characterization of Carbapenemase-Producing Enterobacteriaceae Strains in a Referral Teaching Hospital in Yaoundé, Cameroon  

作　　者：Cecile Ingrid Djuikoue Paule Dana Djouela Djoulako Rodrigue Kamga Wouambo Charlène Nkouankou Tomi Christiane Possi Kiyang Murielle Chantale Tchitchoua Vynnie Manuella Nyatchoutou Blondelle Kitio Messeu Herman Koyouo Tagne Cedric Dylan Seugnou Nana Nadjia Benhamed Hortense Gonsu Kamga Benjamin D. Thumamo Pokam Cecile Ingrid Djuikoue;Paule Dana Djouela Djoulako;Rodrigue Kamga Wouambo;Charlène Nkouankou Tomi;Christiane Possi Kiyang;Murielle Chantale Tchitchoua;Vynnie Manuella Nyatchoutou;Blondelle Kitio Messeu;Herman Koyouo Tagne;Cedric Dylan Seugnou Nana;Nadjia Benhamed;Hortense Gonsu Kamga;Benjamin D. Thumamo Pokam(Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon;Faculty of Medicine, Sorbonne Université, Paris, France;Division of Hepatology, Department of Medecine II, Leipzig University Medical Center, Leipzig, Germany;University of Sciences and Technology, Oran, Algeria;Bacteriology Unit, University Teaching Hospital, Yaounde, Cameroon;Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon)

机构地区：[1]Faculty of Health Sciences, Université des Montagnes, Bangangté, Cameroon [2]Faculty of Medicine, Sorbonne Université, Paris, France [3]Division of Hepatology, Department of Medecine II, Leipzig University Medical Center, Leipzig, Germany [4]University of Sciences and Technology, Oran, Algeria [5]Bacteriology Unit, University Teaching Hospital, Yaounde, Cameroon [6]Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon

出　　处：《Open Journal of Medical Microbiology》2023年第1期52-67,共16页医学微生物学（英文）

摘　　要：Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an important and increasing threat to global health. They are nowadays more encountered routinely in hospitals and cause high morbidity and mortality due to limited therapeutic alternatives. This study sought to determine the prevalence of CPE in Yaoundé teaching hospital, Cameroon, and the associated risk factors. Materials and Method: To achieve this goal, a descriptive cross-sectional study coupled to an analytical component with consecutive collection of Enterobacteria strains was carried out during a three-month period (from 27^th July to 24^th October 2018) in the University Teaching Hospital of Yaoundé, Cameroon. The oxidase and biochemical identification tests using a miniaturized Api 20 E system were performed on colonies grown on Eosin Methylene Blue (EMB) medium and subcultured on nutrient agar. Drug susceptibility testing was carried out according to the Antibiogram Committee of the French Society of Microbiology (CA-SFM 2018.V.2.0). The detection of carbapenemase production was performed by the CA-SFM 2018 algorithm for the screening of carbapenemase-producing enterobacteriaceae and its classification by inhibitory synergy tests. Results: Out of the 104 isolates, Escherichia coli (50%) was the most prevalent species, followed by Klebsiella pneumoniae (37.5%) and Citrobacter frendii (12.5%). Drugs susceptibility patterns showed a high resistance to penicillins group (97.4% to amoxicillin), cephalosporins (68.4% to cefotaxim, 58.1% to cefixim, 60.7% to ceftazidim, 57.1% of cefoxitin) and aztreonam (55.7%). However, 11.9% carbapenems related resistance was noticed: 14.4% to imipenem, 13.8% to ertapenem and 7.5% to meropenem. Numerous co-resistance to quinolones (65.8%), fluoroquinolones (49.6%), aminoglycosides (49.6%) and cotrimoxazole (71.8%) were also observed. From 104 isolates, AmpC production represented 23.08% (25/104) and 36.54% (38/104) were ESBL-isolates. The overall prevalence of CPE was 25% (26/104) with KBackground: Carbapenemase-producing Enterobacteriaceae (CPE) are an important and increasing threat to global health. They are nowadays more encountered routinely in hospitals and cause high morbidity and mortality due to limited therapeutic alternatives. This study sought to determine the prevalence of CPE in Yaoundé teaching hospital, Cameroon, and the associated risk factors. Materials and Method: To achieve this goal, a descriptive cross-sectional study coupled to an analytical component with consecutive collection of Enterobacteria strains was carried out during a three-month period (from 27^th July to 24^th October 2018) in the University Teaching Hospital of Yaoundé, Cameroon. The oxidase and biochemical identification tests using a miniaturized Api 20 E system were performed on colonies grown on Eosin Methylene Blue (EMB) medium and subcultured on nutrient agar. Drug susceptibility testing was carried out according to the Antibiogram Committee of the French Society of Microbiology (CA-SFM 2018.V.2.0). The detection of carbapenemase production was performed by the CA-SFM 2018 algorithm for the screening of carbapenemase-producing enterobacteriaceae and its classification by inhibitory synergy tests. Results: Out of the 104 isolates, Escherichia coli (50%) was the most prevalent species, followed by Klebsiella pneumoniae (37.5%) and Citrobacter frendii (12.5%). Drugs susceptibility patterns showed a high resistance to penicillins group (97.4% to amoxicillin), cephalosporins (68.4% to cefotaxim, 58.1% to cefixim, 60.7% to ceftazidim, 57.1% of cefoxitin) and aztreonam (55.7%). However, 11.9% carbapenems related resistance was noticed: 14.4% to imipenem, 13.8% to ertapenem and 7.5% to meropenem. Numerous co-resistance to quinolones (65.8%), fluoroquinolones (49.6%), aminoglycosides (49.6%) and cotrimoxazole (71.8%) were also observed. From 104 isolates, AmpC production represented 23.08% (25/104) and 36.54% (38/104) were ESBL-isolates. The overall prevalence of CPE was 25% (26/104) with K

关 键 词：ENTEROBACTERIACEAE ANTIBIOTICS CARBAPENEMASE RESISTANCE 

分 类 号：F42[经济管理—产业经济]