Antibiotic Resistance Profile of Serotypes of Streptococcus pneumoniae Strains in Bangui, from 2017 to 2022: Case of Serotype 1  

作　　者：Zéphirin Dalengat Vogbia Ernest Lango Yaya Marceline Djeintote Stéphanie Judith N’Yetobouko Jean de Dieu Longo Clotaire Donatien Rafaï Christian Diamant Mossoro-Kpindet Gérard Gresenguet Zéphirin Dalengat Vogbia;Ernest Lango Yaya;Marceline Djeintote;Stéphanie Judith N’Yetobouko;Jean de Dieu Longo;Clotaire Donatien Rafaï;Christian Diamant Mossoro-Kpindet;Gérard Gresenguet(Epidemiology and Research Service, National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy, Ministry of Health, Bangui, Central African Republic;Faculty of Health Sciences, Doctoral School of Human and Veterinary Health Sciences, University of Bangui, Bangui, Central African Republic;National Laboratory of Clinical Biology and Public Health, Bangui, Central African Republic;National Blood Transfusion Center, Bangui, Central African Republic;Public Department, Ministry of Health, Bangui, Central African Republic)

机构地区：[1]Epidemiology and Research Service, National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy, Ministry of Health, Bangui, Central African Republic [2]Faculty of Health Sciences, Doctoral School of Human and Veterinary Health Sciences, University of Bangui, Bangui, Central African Republic [3]National Laboratory of Clinical Biology and Public Health, Bangui, Central African Republic [4]National Blood Transfusion Center, Bangui, Central African Republic [5]Public Department, Ministry of Health, Bangui, Central African Republic

出　　处：《Open Journal of Medical Microbiology》2024年第2期131-145,共15页医学微生物学（英文）

摘　　要：Goals: The aim of this study was to determine the antibiotic resistance profile of serotypes of Streptococcus pneumoniae strains circulating in Bangui. Methodology: A prospective and analytical analysis was carried out at the National Laboratory of Clinical Biology and Public Health from 2017 to 2022. The strains came from our study on the contribution to the study of antibiotic sensitivity of Streptococcus pneumoniae strains. The multiplex PCR test was used for its cost-effectiveness in terms of amplifiers which can be purified in order to be sequenced. It also makes it possible to detect several germs as well as their serotypes. For a PCR reaction, several elements are involved in the reaction medium or Master Mix. These are the desoxyribonucleotides (dNTPs), the magnesium ions (MgCl2) and the primers. A set of 14 primers divided into 3 classes were used. Class 1 primers served as an internal control by targeting the cpsA gene. It is a highly conserved gene found in capsular loci characterized to date. The primers of the second class were used to target specific serotypes by specific reactions (out of six possibilities). The group reaction was carried out using the primers of the third class in order to carry out an initial screening of the samples and to classify the pneumococcal isolates. Related serotypes were grouped based on the amplification of common genes. Using the technique of electrophoresis on agarose gel and an ultraviolet radiation device, the migration bands are then visualized and analyzed. The data collected had been entered into Excel 2010 and analyzed with Epi info 7. The exact Fischer chi2 test at the 5% threshold, the relative risk and its 95% confidence interval were used to compare the proportions and determine the associations. Results: 187 antibiotic-resistant strains of Streptococcus pneumoniae were collected. The average frequency of serotypes 1, 9A, 4 and untypeable identified were 43.59%, 18.18%, 18.27% and 39.57% respectively. The frequency of serotype 1 was predominant for the ageGoals: The aim of this study was to determine the antibiotic resistance profile of serotypes of Streptococcus pneumoniae strains circulating in Bangui. Methodology: A prospective and analytical analysis was carried out at the National Laboratory of Clinical Biology and Public Health from 2017 to 2022. The strains came from our study on the contribution to the study of antibiotic sensitivity of Streptococcus pneumoniae strains. The multiplex PCR test was used for its cost-effectiveness in terms of amplifiers which can be purified in order to be sequenced. It also makes it possible to detect several germs as well as their serotypes. For a PCR reaction, several elements are involved in the reaction medium or Master Mix. These are the desoxyribonucleotides (dNTPs), the magnesium ions (MgCl2) and the primers. A set of 14 primers divided into 3 classes were used. Class 1 primers served as an internal control by targeting the cpsA gene. It is a highly conserved gene found in capsular loci characterized to date. The primers of the second class were used to target specific serotypes by specific reactions (out of six possibilities). The group reaction was carried out using the primers of the third class in order to carry out an initial screening of the samples and to classify the pneumococcal isolates. Related serotypes were grouped based on the amplification of common genes. Using the technique of electrophoresis on agarose gel and an ultraviolet radiation device, the migration bands are then visualized and analyzed. The data collected had been entered into Excel 2010 and analyzed with Epi info 7. The exact Fischer chi2 test at the 5% threshold, the relative risk and its 95% confidence interval were used to compare the proportions and determine the associations. Results: 187 antibiotic-resistant strains of Streptococcus pneumoniae were collected. The average frequency of serotypes 1, 9A, 4 and untypeable identified were 43.59%, 18.18%, 18.27% and 39.57% respectively. The frequency of serotype 1 was predominant for the age

关 键 词：Streptococcus pneumonia SEROTYPE Antibiotic Resistance Bangui 

分 类 号：R37[医药卫生—病原生物学]