Epidemiology and Clonal Spread Evidence of Carbapenem-Resistant Organisms in the Center of Care and Protection of Orphaned Children, Vietnam  

作　　者：Van Kim Nguyen Pirom Noisumdaeng Pol. Maj. Katiya Ivanovitch Stephen Baker Eugene Athan Stephanie Jones Le Thi Lan Larry Croft Yin Peng Lee Tara Cassidy Van Hung Tran Thi Hang Phan Huu Tinh Ho Van Kim Nguyen;Pirom Noisumdaeng;Pol. Maj. Katiya Ivanovitch;Stephen Baker;Eugene Athan;Stephanie Jones;Le Thi Lan;Larry Croft;Yin Peng Lee;Tara Cassidy;Van Hung Tran;Thi Hang Phan;Huu Tinh Ho(Faculty of Public Health, Thammasat University, Bangkok, Thailand;Department of Prevention and Infection Control, Hung Vuong Hospital, HCM City, Vietnam;Microbiology of Faculty of Public Health, Thammasat University, Bangkok, Thailand;Thammasat University Research Unit in Modern Microbiology and Public Health Genomics, Thammasat University, Bangkok, Thailand;National Heart & Lung Institute (NHLI), University of Cambridge, Cambridge, UK;Barwon South West Public Health Unit, Geelong, Australia;Center for Innovation in Infectious Disease and Immunology Research (CIIDIR), Geelong, Australia;School of Medicine, Deakin University Burwood, Australia;Monash Health, Berwick, Australia;Center of Care and Protection of Orphaned Children, HCM City, Vietnam;Charles River Laboratories, Microbial Solutions, Melbourne, Australia;Personnel Department of Nhan Dan Gia Dinh Hospital, HCM City, Vietnam;Hung Vuong Hospital, HCM City, Vietnam;Department of Epidemiology, Institute of Public Health, HCM City, Vietnam)

机构地区：[1]Faculty of Public Health, Thammasat University, Bangkok, Thailand [2]Department of Prevention and Infection Control, Hung Vuong Hospital, HCM City, Vietnam [3]Microbiology of Faculty of Public Health, Thammasat University, Bangkok, Thailand [4]Thammasat University Research Unit in Modern Microbiology and Public Health Genomics, Thammasat University, Bangkok, Thailand [5]National Heart & Lung Institute (NHLI), University of Cambridge, Cambridge, UK [6]Barwon South West Public Health Unit, Geelong, Australia [7]Center for Innovation in Infectious Disease and Immunology Research (CIIDIR), Geelong, Australia [8]School of Medicine, Deakin University Burwood, Australia [9]Monash Health, Berwick, Australia [10]Center of Care and Protection of Orphaned Children, HCM City, Vietnam [11]Charles River Laboratories, Microbial Solutions, Melbourne, Australia [12]Personnel Department of Nhan Dan Gia Dinh Hospital, HCM City, Vietnam [13]Hung Vuong Hospital, HCM City, Vietnam [14]Department of Epidemiology, Institute of Public Health, HCM City, Vietnam

出　　处：《Open Journal of Medical Microbiology》2024年第3期165-189,共25页医学微生物学（英文）

摘　　要：Objective: To determine the prevalence of colonization and transmission of carbapenem-resistant Gram-negative organisms in order to develop of an effective infection prevention program. Design: Cross-sectional study with carbapenem-resistant organisms (CRO) colonization detection from the fecal specimens of 20 Health Care Workers (HCWs) and 67 residents and 175 random environment specimens from September 2022 to September 2023. Setting: A Care and Protection Centre of Orphaned Children in South of HCM City. Participants: It included 20 HCWs, 67 residents, and 175 randomly collected environmental specimens. Method: Rectal and environmental swabs were collected from 20 HCWs, 67 residents (most of them were children), and 175 environmental specimens. MELAB Chromogenic CARBA agar plates, Card NID, and NMIC-500 CPO of the BD Phoenix TM Automated Microbiology System and whole genome sequencing (WGS) were the tests to screen, confirm CROs, respectively and determine CRO colonization and transmission between HCWs, residents, and the environment. Result: We detected 36 CRO isolates, including 6, 11 and 19 CROs found in 6 HCWs, 10 residents and 19 environments. The prevalence of detectable CRO was 30% (6/20) in HCWs, 14.92% (10/67) in residents, and 10.86% (19/175) in environmental swabs in our study. WGS demonstrated CRO colonization and transmission with the clonal spread of E. coli and A. nosocomialis, among HCWs and residents (children). Conclusion: Significant CRO colonization and transmission was evident in HCWs, residents, and the center environment. Cleaning and disinfection of the environment and performing regular hand hygiene are priorities to reduce the risk of CRO colonization and transmission.Objective: To determine the prevalence of colonization and transmission of carbapenem-resistant Gram-negative organisms in order to develop of an effective infection prevention program. Design: Cross-sectional study with carbapenem-resistant organisms (CRO) colonization detection from the fecal specimens of 20 Health Care Workers (HCWs) and 67 residents and 175 random environment specimens from September 2022 to September 2023. Setting: A Care and Protection Centre of Orphaned Children in South of HCM City. Participants: It included 20 HCWs, 67 residents, and 175 randomly collected environmental specimens. Method: Rectal and environmental swabs were collected from 20 HCWs, 67 residents (most of them were children), and 175 environmental specimens. MELAB Chromogenic CARBA agar plates, Card NID, and NMIC-500 CPO of the BD Phoenix TM Automated Microbiology System and whole genome sequencing (WGS) were the tests to screen, confirm CROs, respectively and determine CRO colonization and transmission between HCWs, residents, and the environment. Result: We detected 36 CRO isolates, including 6, 11 and 19 CROs found in 6 HCWs, 10 residents and 19 environments. The prevalence of detectable CRO was 30% (6/20) in HCWs, 14.92% (10/67) in residents, and 10.86% (19/175) in environmental swabs in our study. WGS demonstrated CRO colonization and transmission with the clonal spread of E. coli and A. nosocomialis, among HCWs and residents (children). Conclusion: Significant CRO colonization and transmission was evident in HCWs, residents, and the center environment. Cleaning and disinfection of the environment and performing regular hand hygiene are priorities to reduce the risk of CRO colonization and transmission.

关 键 词：Carbapenem-Resistant Organisms Contamination Hand Hygiene Whole Genome Sequencing Infection Prevention 

分 类 号：R54[医药卫生—心血管疾病]