Isolation, purification and partial characterization of a glycoside from the Jamaican ball moss plant (<i>Tillandsia recurvata</i>L.)  

作　　者：Henry I. C. Lowe Charah T. Watson Simone Badal Ngeh J. Toyang Joseph Bryant 

机构地区：[1]Bio-Tech R & D Institute, Kingston, Jamaica [2]Institute of Human Virology, School of Medicine, University of Maryland, Baltimore, USA [3]Natural Products Institute, University of the West Indies, Mona, Jamaica

出　　处：《Advances in Biological Chemistry》2012年第3期274-282,共9页生物化学进展（英文）

摘　　要：Initial isolation and characterization screens have been carried out on the Jamaican ball moss (Tillandsia recurvata L.) plant producing a novel complex gly-cosidic compound. This complex glycoside may be responsible for the promising anti-cancer and anti-inflammatory properties of this ball moss plant. A combination of supercritical fluid processing and chromatographic purification was used to produce the isolate. LC-MS analysis showed the presence of a compound with a molecular weight of ~800 and mass spectral (MS-MS and MS-MS-MS) data indicate that this compound may be a glycoside with a molecular mass of 1601.1 Da (negative ionization mode) and possibly contain up to two sialic acids, based on the doubly-charged state of the molecule at neutral pH conditions. 2D-TOCSY NMR spectrum lends evidence to the presence of at least one likely amide to aliphatic proton correlation, indicating the presence of a sialic acid moiety. The main structure of this therapeutic compound appears similar to a soyasaponin.Initial isolation and characterization screens have been carried out on the Jamaican ball moss (Tillandsia recurvata L.) plant producing a novel complex gly-cosidic compound. This complex glycoside may be responsible for the promising anti-cancer and anti-inflammatory properties of this ball moss plant. A combination of supercritical fluid processing and chromatographic purification was used to produce the isolate. LC-MS analysis showed the presence of a compound with a molecular weight of ~800 and mass spectral (MS-MS and MS-MS-MS) data indicate that this compound may be a glycoside with a molecular mass of 1601.1 Da (negative ionization mode) and possibly contain up to two sialic acids, based on the doubly-charged state of the molecule at neutral pH conditions. 2D-TOCSY NMR spectrum lends evidence to the presence of at least one likely amide to aliphatic proton correlation, indicating the presence of a sialic acid moiety. The main structure of this therapeutic compound appears similar to a soyasaponin.

关 键 词：Jamaican BALL MOSS TILLANDSIA recurvata Anti-Cancer Anti-Inflammatory LC-MS High-Resolution Mass Spectrometry Total Correlation Spectroscopy (TOCSY) 

分 类 号：O6[理学—化学]