Comparison of the Biological Activity and Constituents in Japanese Ambers  

作　　者：Eisaku Shimizu Nozomu Shimoda Tetsuaki Kawamura Naomi Ueda Ken-ichi Kimura Eisaku Shimizu;Nozomu Shimoda;Tetsuaki Kawamura;Naomi Ueda;Ken-ichi Kimura(Graduate School of Arts and Sciences, Iwate University, Morioka, Japan;Gangoji Institute for Research of Cultural Property, Nara, Japan)

机构地区：[1]Graduate School of Arts and Sciences, Iwate University, Morioka, Japan [2]Gangoji Institute for Research of Cultural Property, Nara, Japan

出　　处：《Advances in Biological Chemistry》2020年第3期99-112,共14页生物化学进展（英文）

摘　　要：<span style="font-family:Verdana;">Backgrou</span><span style="font-family:Verdana;">nd/Aim: Kuji amber is an interesting natural source for drug discovery</span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">because a new anti-allergic compound, named kujigamberol and several new compounds have been isolatated from it. It was important to evaluate the yield, biological activities and constituents of each methanol extract</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">of Kuji, Iwaki, Choshi, Mizunami and Ube ambers in Japan in order to establish if additional new compounds could be identified in these ambers. Materia</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">ls and </span><span style="font-family:Verdana;">Method: Biological activities of each extract were evaluated using growth-restoring </span><span style="font-family:Verdana;">activity of the mutant yeast strain involving Ca</span>^{<span style="font-family:Verdana;">2+</span>}<span style="font-family:Verdana;">-signal transduction and inhibi</span><span style="font-family:Verdana;">tion activity of degranulation in rat basophilic leukemia (RBL)-2H3 cells. Constituents</span><span style="font-family:Verdana;"> of each extract were analyzed by high performance liquid chromatography (HPLC). Results: All ambers except Ube amber have growth-restoring activity against the mutant yeast. Both Kuji and Iwaki ambers inhibited the degranulation of RBL-2H3 cells induced by the calcium ionophore A23187 in a dose dependent manner. The main biologically active compound in Kuji amber, kujigamberol, was also isolated from Iwaki amber and analyzed by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Conclusion: Kuji and Iwaki ambers app<span style="font-family:Verdana;">Backgrou</span><span style="font-family:Verdana;">nd/Aim: Kuji amber is an interesting natural source for drug discovery</span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">because a new anti-allergic compound, named kujigamberol and several new compounds have been isolatated from it. It was important to evaluate the yield, biological activities and constituents of each methanol extract</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">of Kuji, Iwaki, Choshi, Mizunami and Ube ambers in Japan in order to establish if additional new compounds could be identified in these ambers. Materia</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">ls and </span><span style="font-family:Verdana;">Method: Biological activities of each extract were evaluated using growth-restoring </span><span style="font-family:Verdana;">activity of the mutant yeast strain involving Ca</span>^{<span style="font-family:Verdana;">2+</span>}<span style="font-family:Verdana;">-signal transduction and inhibi</span><span style="font-family:Verdana;">tion activity of degranulation in rat basophilic leukemia (RBL)-2H3 cells. Constituents</span><span style="font-family:Verdana;"> of each extract were analyzed by high performance liquid chromatography (HPLC). Results: All ambers except Ube amber have growth-restoring activity against the mutant yeast. Both Kuji and Iwaki ambers inhibited the degranulation of RBL-2H3 cells induced by the calcium ionophore A23187 in a dose dependent manner. The main biologically active compound in Kuji amber, kujigamberol, was also isolated from Iwaki amber and analyzed by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Conclusion: Kuji and Iwaki ambers app

关 键 词：Japanese Ambers Kujigamberol Growth Restoration of a Mutant Yeast Inhibition of Degranulation RBL-2H3 Cells 

分 类 号：O17[理学—数学]