Radiation-Induced Chromosome Instability in WTK1 and TK6 Human Lymphoblastoid Cells  

作　　者：Alan Chant Heather Driscoll Joshua L. Weiss Ahmad Chaudary Christina M. Kraemer-Chant Alan Chant;Heather Driscoll;Joshua L. Weiss;Ahmad Chaudary;Christina M. Kraemer-Chant(Department of Chemistry, Saint Michael’s College, Colchester, USA;Department of Biology, Norwich University, Northfield, USA;Department of Public and Allied Health, Bowling Green State University, Bowling Green, USA)

机构地区：[1]Department of Chemistry, Saint Michael’s College, Colchester, USA [2]Department of Biology, Norwich University, Northfield, USA [3]Department of Public and Allied Health, Bowling Green State University, Bowling Green, USA

出　　处：《Advances in Biological Chemistry》2023年第2期57-70,共14页生物化学进展（英文）

摘　　要：The effects of ionizing radiation on single nucleotide polymorphism (SNP) copy number variations between TK6 and WTK1 cell lines are described herein. Specifically, the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) was analyzed in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated post-irradiation for SNP analysis, which showed significant, genome-wide impacts on both cell lines;for the mutant WTK1 sample, there were a total of 48 gene deletions and no gene amplifications, whereas for the wild-type TK6 sample, there were 217 gene deletions and 9 gene amplifications. It appears that both cell lines are affected in the areas of cell-cycle control, but that other affected areas differ significantly between the two.The effects of ionizing radiation on single nucleotide polymorphism (SNP) copy number variations between TK6 and WTK1 cell lines are described herein. Specifically, the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) was analyzed in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated post-irradiation for SNP analysis, which showed significant, genome-wide impacts on both cell lines;for the mutant WTK1 sample, there were a total of 48 gene deletions and no gene amplifications, whereas for the wild-type TK6 sample, there were 217 gene deletions and 9 gene amplifications. It appears that both cell lines are affected in the areas of cell-cycle control, but that other affected areas differ significantly between the two.

关 键 词：Ionizing Radiation Single Nucleotide Polymorphism p53 Tumor Suppressor 

分 类 号：R11[医药卫生—公共卫生与预防医学]