Sequential Injection Analysis of Ampicillin in Pharmaceuticals by Using Potentiometric Detectors Based on PVC and Sol-Gel Membranes  

作　　者：Nestor Zarate Alberto N.Araujo M.Conceicao B.S.M.Montenegro Ricardo Perez-Olmos 

机构地区：[1]Dpto.Quimica Analítica,Escuela Universitaria de Ingenieria Tecnica Industrial,Universidad del Pais Vasco,Plaza de la Casilla 3,48012,Bilbao [2]REQUIMTE,Dpto.de Quimica Fisica,Faculdade de Farmacia,Universidade do Porto,Rua Anibal Cunha 164,4050 Porto,Portugal

出　　处：《American Journal of Analytical Chemistry》2011年第4期491-499,共9页美国分析化学（英文）

基　　金：supported by the University of the Basque Country(Spain)through Project UPV 171.363-E-15923/2004;this University for the Ph.D.Grant 98/13828/01.

摘　　要：This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, incorporated in both PVC and sol-gel membranes and directly applied onto a conductive graphite/epoxy resin support. The units were constructed without inner reference solution adopting conventional configuration and in the case of PVC membrane the tubular configuration was also adopted. The good working characteristics of these electrodes, made possible its application to the determination of ampicillin in pharmaceuticals formulations, both in conventional batch analysis and in flow conditions, when the electrodes were coupled to a SIA system. In the last case the potentiometric sensors presented linear response towards ampicillin concentration between 5.0 × 10–4 and 5.0 × 10–2 mol?l–1 with slopes of –57.4 and –63.5 mV?dec–1 for the PVC and sol-gel membranes, respectively. The developed procedures enable mean relative standard deviations better than 3% for all the samples analysed. The obtained results do not statistically differ from those furnished by applying the HPLC reference method.This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, incorporated in both PVC and sol-gel membranes and directly applied onto a conductive graphite/epoxy resin support. The units were constructed without inner reference solution adopting conventional configuration and in the case of PVC membrane the tubular configuration was also adopted. The good working characteristics of these electrodes, made possible its application to the determination of ampicillin in pharmaceuticals formulations, both in conventional batch analysis and in flow conditions, when the electrodes were coupled to a SIA system. In the last case the potentiometric sensors presented linear response towards ampicillin concentration between 5.0 × 10–4 and 5.0 × 10–2 mol?l–1 with slopes of –57.4 and –63.5 mV?dec–1 for the PVC and sol-gel membranes, respectively. The developed procedures enable mean relative standard deviations better than 3% for all the samples analysed. The obtained results do not statistically differ from those furnished by applying the HPLC reference method.

关 键 词：AMPICILLIN SIA Potentiometric Detection Pharmaceutical Formulations 

分 类 号：O6[理学—化学]