High Resolution Mass Spectrometry Elucidation of Captopril’s Ozonation and Chlorination By-Products  

作　　者：Frederico Jehar Oliveira Quintao Geraldo Celio Brandao Silvana de Queiroz Silva Sergio Francisco Aquino Robson Jose de Cassia Franco Afonso 

机构地区：[1]Programa de Pos-Graduacao em Engenharia Ambiental(ProAmb),Universidade Federal de Ouro Preto,Ouro Preto,Brazil [2]Programa de Pos-Graduacao em Ciencias Farmaceuticas(CiPharma),Universidade Federal de Ouro Preto,Ouro Preto,Brazil

出　　处：《American Journal of Analytical Chemistry》2017年第4期264-279,共16页美国分析化学（英文）

基　　金：the Minas Gerais State Science Foundation(FAPEMIG)-process APQ-00918-13;the Brazilian National Research Council(CNPq)-process 473893-2013-1 for their financial support and the granting of research fellowships.

摘　　要：The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of its by-products by applying direct infusion and high performance liquid chromatography, electrospray ionization high resolution mass spectrometry, in the negative ion mode. The cytotoxicity of its by-products solutions were evaluated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. It was observed through that after 30 min of ozonation and chlorination, there was complete oxidation of captopril, i.e., 100% removal efficiency. At these conditions, the rate of mineralization, by total organic carbon, was only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation of degradation by-products. Ten captopril by-products were identified and their respective chemical structures elucidations are proposed. The treated samples and their by-products were nontoxic to HepG2 cells by MTT assay.The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of its by-products by applying direct infusion and high performance liquid chromatography, electrospray ionization high resolution mass spectrometry, in the negative ion mode. The cytotoxicity of its by-products solutions were evaluated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. It was observed through that after 30 min of ozonation and chlorination, there was complete oxidation of captopril, i.e., 100% removal efficiency. At these conditions, the rate of mineralization, by total organic carbon, was only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation of degradation by-products. Ten captopril by-products were identified and their respective chemical structures elucidations are proposed. The treated samples and their by-products were nontoxic to HepG2 cells by MTT assay.

关 键 词：CHLORINATION OZONATION CAPTOPRIL High-Resolution Mass Spectrometry Liquid Chromatography Characterization of By-Products MTT Assay 

分 类 号：O6[理学—化学]