Phytochemical Tests, Assessment of Antioxidant Properties and Isolation of Two Compounds of Ethyl Acetate Extract of Chadian Propolis: Case of Bebotho (Southern Chad)  

作　　者：Salomon Madjitoloum Betoloum Abel Mbaiogaoun Severin Mbaihougadobe Emmanuel Talla Salomon Madjitoloum Betoloum;Abel Mbaiogaoun;Severin Mbaihougadobe;Emmanuel Talla(Department of Chemistry, Faculty of Exact and Applied Sciences (FSEA), University of N’Djamena, N’Djamena, The Republic of Chad;Department of Chemistry, Faculty of Science, University of Ngaoundéré (UN), Ngaoundéré, The Republic of Cameroon)

机构地区：[1]Department of Chemistry, Faculty of Exact and Applied Sciences (FSEA), University of N’Djamena, N’Djamena, The Republic of Chad [2]Department of Chemistry, Faculty of Science, University of Ngaoundéré (UN), Ngaoundéré, The Republic of Cameroon

出　　处：《American Journal of Analytical Chemistry》2022年第7期241-254,共14页美国分析化学（英文）

摘　　要：The present work deals with the research of chemical constituents and evaluation of antioxidant properties of Bebotho propolis. From the ethyl acetate extract, we isolated, using various chromatographic techniques, a mixture of two identical compounds (isomers) indexed PBy4a and PByb. The structures of these compounds were elucidated by means of spectroscopic analysis techniques (MS, IR, ¹H-NMR, ¹³C-NMR, HMBC and HSQC) and by comparison of the spectral data with those described in the literature. Thus, these compounds were identified to a mixture of two chromones namely 5,7-dihydroxy-2-methylchromone-6-C-α-D-glucopyranoside and 5,7-dihydroxy-2-methylchromone-8-C-β-D-glucopyranoside, first reported in propolis. The study of the antiradical power, chelating power and the quantification of phenolic compound of these same extracts, showed interesting properties that propolis extracts have to scavenge free radicals.The present work deals with the research of chemical constituents and evaluation of antioxidant properties of Bebotho propolis. From the ethyl acetate extract, we isolated, using various chromatographic techniques, a mixture of two identical compounds (isomers) indexed PBy4a and PByb. The structures of these compounds were elucidated by means of spectroscopic analysis techniques (MS, IR, ¹H-NMR, ¹³C-NMR, HMBC and HSQC) and by comparison of the spectral data with those described in the literature. Thus, these compounds were identified to a mixture of two chromones namely 5,7-dihydroxy-2-methylchromone-6-C-α-D-glucopyranoside and 5,7-dihydroxy-2-methylchromone-8-C-β-D-glucopyranoside, first reported in propolis. The study of the antiradical power, chelating power and the quantification of phenolic compound of these same extracts, showed interesting properties that propolis extracts have to scavenge free radicals.

关 键 词：ISOLATION PROPOLIS Chelating Power 5 7-Dihydroxy-2-Methylchromone-6-C-α-D-Glucopyranoside and 5 7-Dihydroxy-2-Methylchromone-8-C-β-D-Glucopyranoside 

分 类 号：O62[理学—有机化学]