Chiral Method Development Using Polysaccharide-Based Chiral Stationary Phase (CSP) and Investigation of Chiral Column Degradation and Regeneration  

作　　者：Hua Zhao Mary Ababat Kyle Leeman John Tucker Hua Zhao;Mary Ababat;Kyle Leeman;John Tucker(Neurocrine Biosciences INC., San Diego, CA, USA;Mirati Therapeutics, San Diego, CA, USA)

机构地区：[1]Neurocrine Biosciences INC., San Diego, CA, USA [2]Mirati Therapeutics, San Diego, CA, USA

出　　处：《American Journal of Analytical Chemistry》2024年第12期395-406,共12页美国分析化学(英文)

摘　　要：Two polysaccharide-based chiral stationary phase columns were evaluated to improve the previous partial chiral peak separation to a baseline-resolved separation of the INGREZZA® drug substance and its diastereomers. Moreover, the tailing factor (Tf) variation was studied to investigate chiral column degradation and regeneration and to optimize chiral column performance and efficiency.Two polysaccharide-based chiral stationary phase columns were evaluated to improve the previous partial chiral peak separation to a baseline-resolved separation of the INGREZZA® drug substance and its diastereomers. Moreover, the tailing factor (Tf) variation was studied to investigate chiral column degradation and regeneration and to optimize chiral column performance and efficiency.

关 键 词：Chiral Reversed-Phase HPLC Separation Backflush Chiral Column Regeneration INGREZZA® (Valbenazine) 

分 类 号：O62[理学—有机化学]