Validation of a Method for Characterization of Ethanol in Water by HS-GC-FID to Serve the Traceability of Halal Measurements  

作　　者：Adel B. Shehata Abdulrahman R. Al Askar Mohammed A. Al Rasheed Abdulrahman M. Al Zahrany Fahd A. Al Kharraa Sowailem A. Al Sowailem Adel B. Shehata;Abdulrahman R. Al Askar;Mohammed A. Al Rasheed;Abdulrahman M. Al Zahrany;Fahd A. Al Kharraa;Sowailem A. Al Sowailem(Chemistry Department/National Measurement and Calibration Center (NMCC), Saudi Standards, Metrology and Quality Organization (SASO), Riyadh, Kingdom of Saudi Arabia)

机构地区：[1]Chemistry Department/National Measurement and Calibration Center (NMCC), Saudi Standards, Metrology and Quality Organization (SASO), Riyadh, Kingdom of Saudi Arabia

出　　处：《Green and Sustainable Chemistry》2024年第2期17-28,共12页绿色与可持续化学（英文）

摘　　要：The determination of the ethanol content in food products is of fundamental importance for HALAL certification. In this work, an analytical method for the determination of ethanol in water by headspace gas chromatography with flame ionization detector (HS-GC-FID) has been developed and validated for the use in characterization of ethanol reference materials. The validation study was carried out in the linear calibration range 100 - 1500 mg/kg using the NIST SRM 2900, nominal 95.6%. The studied performance characteristics of the method were the limit of detection, LOD, the limit of quantification LOQ, selectivity, linearity, precision, recovery and bias. The validation results showed that the method is selective, precise, accurate and free from any significant bias. The LOD and LOQ were 1.27 and 3.86 mg/kg respectively and the estimated expanded uncertainty was 2% indicating that the method is fit for the purpose of certification of ethanol in water reference materials.The determination of the ethanol content in food products is of fundamental importance for HALAL certification. In this work, an analytical method for the determination of ethanol in water by headspace gas chromatography with flame ionization detector (HS-GC-FID) has been developed and validated for the use in characterization of ethanol reference materials. The validation study was carried out in the linear calibration range 100 - 1500 mg/kg using the NIST SRM 2900, nominal 95.6%. The studied performance characteristics of the method were the limit of detection, LOD, the limit of quantification LOQ, selectivity, linearity, precision, recovery and bias. The validation results showed that the method is selective, precise, accurate and free from any significant bias. The LOD and LOQ were 1.27 and 3.86 mg/kg respectively and the estimated expanded uncertainty was 2% indicating that the method is fit for the purpose of certification of ethanol in water reference materials.

关 键 词：ETHANOL HS-GC-FID VALIDATION LINEARITY Precision Bias 

分 类 号：O62[理学—有机化学]