Estimation of Total Phenolic Compounds and Non-Targeted Volatile Metabolomics in Leaf Tissues of American Chestnut (<i>Castanea dentata</i>), Chinese Chestnut (<i>Castanea mollissima</i>) and the Backcross Breeding Generations  

作　　者：Jinyan She Chathuri U. G. Mohottige Mary King Yi Jiang Matt Mlsna Stacy Clark Richard Baird Todd Mlsna Jinyan She;Chathuri U. G. Mohottige;Mary King;Yi Jiang;Matt Mlsna;Stacy Clark;Richard Baird;Todd Mlsna(Department of Chemistry, Mississippi State University, Starkville, USA;Department of Civil and Environmental Engineering, Mississippi State University, Starkville, USA;Department of Agriculture, Forest Service, Southern Research Station, Knoxville, USA;Department of Biochemistry, Molecular Biology, Entomology & Plant Pathology, Mississippi State University, Starkville, USA)

机构地区：[1]Department of Chemistry, Mississippi State University, Starkville, USA [2]Department of Civil and Environmental Engineering, Mississippi State University, Starkville, USA [3]Department of Agriculture, Forest Service, Southern Research Station, Knoxville, USA [4]Department of Biochemistry, Molecular Biology, Entomology & Plant Pathology, Mississippi State University, Starkville, USA

出　　处：《Journal of Agricultural Chemistry and Environment》2021年第2期222-256,共35页农业化学和环境（英文）

摘　　要：The American chestnut (<em>Castanea dentata</em>) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by chestnut blight caused by the Ascomycetous fungus <em>Cryphonectria parasitica</em>. Identification of compounds specific to species and backcross hybrids may help further refine disease resistance breeding and testing. Phenolic compounds produced by plants are significant to their defense mechanisms against fungal pathogens. Therefore, an analytical platform has been developed to estimate the total phenolic content in leaf tissues of the American chestnut, Chinese chestnut (<em>Castanea mollissima</em>), and their backcross breeding generations (B₃F₂ and B₃F₃) using the Folin-Ciocalteu reagent assay with UV/Vis spectrophotometry which may be used to predict blight resistance. Adsorption (765 nm) results from leaf tissue extraction in methanol/water (95%:5% v/v) and pH 2, show that the variations among these four tree species are significant (ANOVA p = 2.3 × 10^-7). The kinetics of phenolic compound solid-liquid extraction was elaborated using Peleg, second order, Elovich, and power law models. In addition, extensive analysis using headspace solid phase microextraction (SPME) gas chromatography and mass spectrometry was conducted to identify volatile organic compounds (VOCs) from the leaf of American chestnut, Chinese chestnut, and their backcross hybrids B₃F₂ and B₃F₃. A total of 67 VOCs were identified among all chestnut types. Many of the metabolites associated with the Chinese chestnut have been reported to have antifungal properties, whereas the native and hybrid American chestnut metabolites have not. Most of the antifungal metabolites showed the strongest efficacy towards the Ascomycota phylum. A partial least squares discriminant analysis (PLS-DA) model (R²X = 0.884, R²Y = 0.917, Q<sup>2<The American chestnut (<em>Castanea dentata</em>) was once a dominant tree species in the Appalachian Mountains and played a critical role in the ecological system. However, it was nearly eliminated by chestnut blight caused by the Ascomycetous fungus <em>Cryphonectria parasitica</em>. Identification of compounds specific to species and backcross hybrids may help further refine disease resistance breeding and testing. Phenolic compounds produced by plants are significant to their defense mechanisms against fungal pathogens. Therefore, an analytical platform has been developed to estimate the total phenolic content in leaf tissues of the American chestnut, Chinese chestnut (<em>Castanea mollissima</em>), and their backcross breeding generations (B₃F₂ and B₃F₃) using the Folin-Ciocalteu reagent assay with UV/Vis spectrophotometry which may be used to predict blight resistance. Adsorption (765 nm) results from leaf tissue extraction in methanol/water (95%:5% v/v) and pH 2, show that the variations among these four tree species are significant (ANOVA p = 2.3 × 10^-7). The kinetics of phenolic compound solid-liquid extraction was elaborated using Peleg, second order, Elovich, and power law models. In addition, extensive analysis using headspace solid phase microextraction (SPME) gas chromatography and mass spectrometry was conducted to identify volatile organic compounds (VOCs) from the leaf of American chestnut, Chinese chestnut, and their backcross hybrids B₃F₂ and B₃F₃. A total of 67 VOCs were identified among all chestnut types. Many of the metabolites associated with the Chinese chestnut have been reported to have antifungal properties, whereas the native and hybrid American chestnut metabolites have not. Most of the antifungal metabolites showed the strongest efficacy towards the Ascomycota phylum. A partial least squares discriminant analysis (PLS-DA) model (R²X = 0.884, R²Y = 0.917, Q<sup>2<

关 键 词：Total Phenolic Compounds Plant VOCs HS-SPME-GC/MS Chemometric Analysis Chestnut Tree Hybrids 

分 类 号：O62[理学—有机化学]