Heat-Resistant Properties of <i>&#945</i>-Chymotrypsin Adsorbed onto Biomass Charcoal Powder  

作　　者：Hidetaka Noritomi Shunichi Kurihara Nobuyuki Endo Satoru Kato 

机构地区：[1]Department of Applied Chemistry, Tokyo Metropolitan University, Tokyo, Japan [2]Department of Applied Chemistry, Tokyo Metropolitan University, Tokyo, Japan 2EEN Co., Ltd., Tokyo, Japan [3]EEN Co., Ltd., Tokyo, Japan

出　　处：《Journal of Biomaterials and Nanobiotechnology》2014年第3期179-185,共7页生物材料与纳米技术（英文）

摘　　要：Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.

关 键 词：Adsorption BIOMASS Charcoal Powder Α-CHYMOTRYPSIN Activity Thermal Stability 

分 类 号：O6[理学—化学]