Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces  

作　　者：Herin Oh Alec Cerchiari Desirée Sorensen Timothy Mon Cassandra L.Smith 

机构地区：[1]Molecular Biology Research Laboratory,Boston University,Boston,USA [2]Department of Biomedical Engineering,Boston Uni-versity,Boston,USA [3]Department of Biology,Boston University,Boston,USA

出　　处：《Materials Sciences and Applications》2013年第9期538-548,共11页材料科学与应用期刊（英文）

摘　　要：Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.

关 键 词：Cyclic Polyolefin Ligation Mediated Rolling Circle Amplification SNP Detection 

分 类 号：R5[医药卫生—内科学]