Isolation of Picocyanobacteria (Order Synechococcales) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond  

作　　者：Nancy J. Leland Kimberley Crocker Pearson Marty K. Burke Jeffrey T. Miller Alison Watts James F. Haney Nancy J. Leland;Kimberley Crocker Pearson;Marty K. Burke;Jeffrey T. Miller;Alison Watts;James F. Haney(Department of Biological Sciences, University of New Hampshire, Center for Freshwater Biology and Ecotoxicology, Durham, New Hampshire, USA;Lim-Tex, North Andover, Massachusetts, USA;Brewster Natural Resources Advisory Commission, Brewster, Massachusetts, USA;Brewster Ponds Coalition, Brewster, Massachusetts, USA;Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA)

机构地区：[1]Department of Biological Sciences, University of New Hampshire, Center for Freshwater Biology and Ecotoxicology, Durham, New Hampshire, USA [2]Lim-Tex, North Andover, Massachusetts, USA [3]Brewster Natural Resources Advisory Commission, Brewster, Massachusetts, USA [4]Brewster Ponds Coalition, Brewster, Massachusetts, USA [5]Department of Civil and Environmental Engineering, University of New Hampshire, College of Engineering and Physical Sciences, Durham, New Hampshire, USA

出　　处：《Journal of Water Resource and Protection》2023年第6期299-314,共16页水资源与保护（英文）

摘　　要：We have verified the use of a serial filtration method to isolate picocyanobacteria for analysis. We used eDNA metabarcoding to confirm the picocyanobacteria as members of the Order Synechococcales, Genus Cyanobium, specifically Cyanobium 6307. Fluorometric analysis using accessory pigments phycocyanin and phycoerythrin described periods of excess biomass, where the net growth rate model confirmed these conditions. The total anatoxin-a concentrations in the picocyanobacterial sample ranged from 0.0074 - 6.41 μg·L^-1 representing a 40-fold difference over the entire sampling season. Sampling frequency of every three days appeared to be an important factor in capturing these changes in anatoxin-a concentration. During a period of excess biomass, we were able to establish a linear correlation between cyanobacterial biomass and Anatoxin-a concentrations.We have verified the use of a serial filtration method to isolate picocyanobacteria for analysis. We used eDNA metabarcoding to confirm the picocyanobacteria as members of the Order Synechococcales, Genus Cyanobium, specifically Cyanobium 6307. Fluorometric analysis using accessory pigments phycocyanin and phycoerythrin described periods of excess biomass, where the net growth rate model confirmed these conditions. The total anatoxin-a concentrations in the picocyanobacterial sample ranged from 0.0074 - 6.41 μg·L^-1 representing a 40-fold difference over the entire sampling season. Sampling frequency of every three days appeared to be an important factor in capturing these changes in anatoxin-a concentration. During a period of excess biomass, we were able to establish a linear correlation between cyanobacterial biomass and Anatoxin-a concentrations.

关 键 词：PICOCYANOBACTERIA ANATOXIN-A EDNA PHYCOCYANIN PHYCOERYTHRIN 

分 类 号：R99[医药卫生—毒理学]