Microcontroller-Based Sinusoidal Voltage Generation for Electrical Bio-Impedance Spectroscopy Applications  

作　　者：Juan A. Castro A. Olmo Pablo Pérez A. Yúfera Juan A. Castro;A. Olmo;Pablo Pérez;A. Yúfera(Departamento de Tecnología Electrónica, Universidad de Sevilla, Seville, Spain;Instituto de Microelectrónica de Sevilla, CSIC, Sevilla, Spain)

机构地区：[1]Departamento de Tecnología Electrónica, Universidad de Sevilla, Seville, Spain [2]Instituto de Microelectrónica de Sevilla, CSIC, Sevilla, Spain

出　　处：《Journal of Computer and Communications》2016年第17期51-58,共8页电脑和通信（英文）

摘　　要：A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC Arduino^TMUno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC Arduino^TMUno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.

关 键 词：Sinusoidal Voltage Generator Electrical Cell-Substrate Impedance Spectroscopy (ECIS) BIOIMPEDANCE Microcontroller (µC) Total Harmonic Distortion (THD) 

分 类 号：TN9[电子电信—信息与通信工程]