Applicability of Dimedone Assays for the Development of Online Aldehyde Sensor in Seawater Flooding Systems  

作　　者：Mohammed A. Al-Moniee Cees Koopal Naim Akmal Sjaak van Veen Xiangyang Zhu Peter F. Sanders Peter F. Sanders Fahad N. Al-Abeedi Ayman M. Amer Mohammed A. Al-Moniee;Cees Koopal;Naim Akmal;Sjaak van Veen;Xiangyang Zhu;Peter F. Sanders;Peter F. Sanders;Fahad N. Al-Abeedi;Ayman M. Amer(Saudi Arabian Oil Company (Saudi Aramco), Dhahran, KSA;TNO (Netherlands Organization for Applied Scientific Research), Zeist, The Netherlands)

机构地区：[1]Saudi Arabian Oil Company (Saudi Aramco), Dhahran, KSA [2]TNO (Netherlands Organization for Applied Scientific Research), Zeist, The Netherlands

出　　处：《Journal of Sensor Technology》2016年第4期101-109,共10页传感技术（英文）

摘　　要：Biocides are oilfield chemicals that are widely used to control bacterial activity throughout the oil industry. A feasibility study has been explored to develop detection techniques for biocide batch treatments, preferably on-line and in real-time, for their potential use in seawater flooding system. Several methods to measure key components of the biocide formulation were investigated and reported in previous study [1]. The enzymatic activity of an immobilized acetylcholine esterase (AChE) on the column material was successfully inhibited by some model compounds, but not by the actual biocides commonly used in Saudi Aramco seawater flooding system. In this paper, an alternative assay for biocide detection in the Saudi Aramco seawater flooding system was investigated for its applicability for the development of on-line biocide sensor. The assay was based on the detection of aldehyde functionality in the biocide mixture through measurement of a fluorescent derivative formed in the reaction of aldehyde groups and dimedone in the presence of ammonium acetate. The reaction of aldehyde groups with dimedone was demonstrated in seawater matrix, and the formed fluorescent product was successfully measured. The results showed that the dimedone-based assay was very sensitive, and relatively straightforward to perform. The ruggedness test also indicated that the assay is sensitive to minor changes of various specific conditions of the method. It is concluded that the dimedone assay is suitable for further development of a real-time biocide monitoring system to detect the presence of biocide slugs in seawater flooding system. The development of an automated on-line biocide sensor based on dimedone assay is underway.Biocides are oilfield chemicals that are widely used to control bacterial activity throughout the oil industry. A feasibility study has been explored to develop detection techniques for biocide batch treatments, preferably on-line and in real-time, for their potential use in seawater flooding system. Several methods to measure key components of the biocide formulation were investigated and reported in previous study [1]. The enzymatic activity of an immobilized acetylcholine esterase (AChE) on the column material was successfully inhibited by some model compounds, but not by the actual biocides commonly used in Saudi Aramco seawater flooding system. In this paper, an alternative assay for biocide detection in the Saudi Aramco seawater flooding system was investigated for its applicability for the development of on-line biocide sensor. The assay was based on the detection of aldehyde functionality in the biocide mixture through measurement of a fluorescent derivative formed in the reaction of aldehyde groups and dimedone in the presence of ammonium acetate. The reaction of aldehyde groups with dimedone was demonstrated in seawater matrix, and the formed fluorescent product was successfully measured. The results showed that the dimedone-based assay was very sensitive, and relatively straightforward to perform. The ruggedness test also indicated that the assay is sensitive to minor changes of various specific conditions of the method. It is concluded that the dimedone assay is suitable for further development of a real-time biocide monitoring system to detect the presence of biocide slugs in seawater flooding system. The development of an automated on-line biocide sensor based on dimedone assay is underway.

关 键 词：DNA Staining Automated Monitoring Autonomous Microbe Sensor Microbial Sensor Prototype Injection Seawater 

分 类 号：O62[理学—有机化学]