钛颗粒诱导小鼠植骨气囊骨溶解模型的构建  

Establishment of Murine Model with Titanium Particles-induced Osteolysis

作　　者：陈德胜[1] 李燕[2] 郭凤英[2] 朱禧[1] 杨旭[2] 张佳林[1] 

机构地区：[1]宁夏医科大学总医院,银川750004 [2]宁夏医科大学,银川750004

出　　处：《宁夏医科大学学报》2016年第11期-,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81560364);宁夏自然科学基金(NZ14114;NZ15081)

摘　　要：目的建立小鼠钛颗粒诱导骨溶解动物模型。方法 30只雄性BALB/c小鼠随机分为对照组和钛颗粒刺激组,每组15只。采用手术方法建立植骨气囊的动物模型,钛颗粒刺激组注射0.3 m L钛颗粒,对照组注射0.3 m L PBS液。气囊植入颅骨骨块14d后取出颅骨及其周围囊壁组织,分别进行大体、组织病理学及超微结构观察。结果大体观察钛颗粒刺激组小鼠植骨骨块与气囊组织之间出现红肿、渗出并伴有新生血管增生,炎性反应明显;对照组的炎性反应较轻。组织病理学见钛颗粒刺激组植入气囊内的骨块表面出现骨吸收现象,并且有明显的炎性反应;对照组无明显骨表面骨吸收的改变,炎性反应较轻。超微结构观察钛颗粒刺激组细胞胞浆内出现钛颗粒浸润,细胞胞浆内高尔基体生长活跃,植骨气囊周围可见增生活跃的破骨细胞前体细胞;对照组破骨细胞内细胞器完整,基质比较丰富。结论在钛颗粒刺激下建立磨损颗粒诱导骨溶解动物模型,可为人工关节无菌性松动导致的假体周围骨溶解的研究提供有效的实验基础。Objective To establish osteolysis animal models induced by titanium particles in mice. Methods 30 male BALB/c mice were randomly divided into control group and titanium particles-stimulated group and each group had 15 mice. Surgical method was adopted to establish the animal model of bone-implanted pouches. In the titanium particles-stimulated group,0.3m L titanium particles was injected to stimulate the inflammatory response and dissolved bone response. In the control group,0.3m L sterile fluid of PBS was injected. Bone-implanted in the skull to be put to death in mice after 14 d, the animal model of bone-implanted pouches were examined respectively by gross,histopathological and ultrastructural observation. Results In the titanium particles-stimulated group,inflammatory reaction was significantly appeared,oozy accompanied by new blood vessel growth in the block between titanium particles stimulate implantation bone air pouches. In the control group,inflammatory reaction was lighter. By histopathological observation,it was obvious that bone resorption and inflammatory reaction in the titanium particles-stimulated group changed. However,bone-implanted pouches in the control group was integrated and the change of bone resorption was not obvious. With ultrastructure observation,cell cytoplasm with titanium particles were infiltration,intracytoplasmic golgi body active growth,and there were hyperplasia of bone graft with active osteoclast precursor cells. In the control group,osteoclast in mitochondria were found. Conclusion Bone dissolve model induced by titanium particles under the stimulus of wear particle can effectively set up the experimental basis that aseptic loosening of artificial joint associated with osteolysis around implant.

关 键 词：骨溶解 动物模型 磨损颗粒 钛颗粒 

分 类 号：R687.4[医药卫生—骨科学] R-332[医药卫生—外科学]