This work was supported by grants from the Ministry of Science and Technology of China (No. 2011CB911102) and the National Natural Science Foundation of China (No. 31100528).
Human GDP-L-fucose synthase, also known as FX protein, synthesizes GDP-L-fucose from its substrate GDP-4-keto-6- deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent ...