高等学校学科创新引智计划(111-2-06)

作品数:54被引量:135H指数:6
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相关作者:张荣珍聂尧郑飞云李崎刘春凤更多>>
相关机构:江南大学教育部怀化学院宿迁市江南大学产业技术研究院更多>>
相关期刊:《Chinese Journal of Natural Medicines》《微生物学通报》《食品与发酵工业》《食品工业科技》更多>>
相关主题:羰基还原酶葡萄糖脱氢酶微生物大肠杆菌SYNTHESIS更多>>
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Development of surface displaying system for heterologous protein expression in Candida tropicalis
《Systems Microbiology and Biomanufacturing》2023年第2期298-311,共14页Tingting Wang Haibing Zhang Mengxuan Li Jiaxi Zheng Yuanyuan Xia Haiquan Yang Wei Shen Xianzhong Chen 
supported by the 111 Project(No.111-2-06);National Natural Science Foundation of China(32001064);Key Research and Development Program of China(2021YFC2100102-03);China Postdoctoral Science Foundation(2020M671331);Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX20-1807).
The purpose of this study was to assess the potential application of cell surface display in Candida tropicalis.Surface display gene cassettes were constructed using five anchoring proteins from Saccharomyces cerevisi...
关键词:Yeast surface display Anchoring protein Glycosylphosphatidylinositol(GPI) Α-AMYLASE Β-GLUCOSIDASE Whole cell catalysis 
Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function被引量:1
《Synthetic and Systems Biotechnology》2023年第2期262-272,共11页Wei-lai Fu Pei-feng Duan Qiang Wang Ya-xin Liao Yun-shuang Wang Mei-juan Xu Hui-hui Jiang Xian Zhang Zhi-ming Rao 
supported by Foundation of Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control(No.FACE20200003);111 Project(111-2-06)and Jiangsu province“Collaborative Innovation Center for Advanced Industrial Fermentation”industry development program.
The biological treatment of wastewater with high concentrations of ammonia nitrogen has become a hot research issue,but there are limited reports on the mechanism of ammonia nitrogen utilization by microorganisms.In t...
关键词:Ammonia nitrogen Pseudomonas stutzeri F2 TRANSCRIPTOMIC ASSIMILATION AMTB 
Synthesis and immunological study of a glycosylated wall teichoic acid-based vaccine against Staphylococcus aureus
《Chinese Chemical Letters》2023年第4期193-197,共5页Peng Shen Han Lin Yikai Bao Haofei Hong Zhimeng Wu 
supported by the National Natural Science Foundation of China (Nos. 21472070 and 22177040);partly supported by the 111 Project (No. 111-2-06)。
Staphylococcus aureus wall teichoic acids(WTAs) are attractive targets for antibacterial vaccine development. In this study, three core glycosylated WTA structure, including α-1,4-Glc NAc, β-1,4-Glc NAc andβ-1,3-Gl...
关键词:WTAs Staphylococcus aureus GlcNAc modifications GLYCOCONJUGATES Antibacterial vaccine 
A CRISPR-Cas12a system for multi-gene editing (CCMGE) and metabolic pathway assembly in Starmerella bombicola
《Systems Microbiology and Biomanufacturing》2022年第4期665-675,共11页Min Zhang Yibo Shi Lihua Zhang Shiying Zhu Haiquan Yang Wei Shen Yuanyuan Xia Xianzhong Chen 
The work was supported by the 111 Project(No.111-2-06);National Natural Science Foundation of China(32001064);Key Research and Development Program of China(2021YFC2100102-03);China Postdoctoral Science Foundation(2020M671331);Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX20-1807).
The traditional homologous recombination(HR)gene-editing method faces problems such as low editing efficiency and absence of marker genes.CRISPR-Cas9-editing efficiency is high and has been widely used in bacteria and...
关键词:Starmerella bombicola CRISPR-Cas12a Genome editing PHA 
MUC1 vaccines usingβ-cyclodextrin grafted chitosan(CS-g-CD)as carrier via host-guest interaction elicit robust immune responses
《Chinese Chemical Letters》2022年第11期4882-4885,共4页Hangyan Yu Han Lin Yuntian Xie Mengyuan Qu Min Jiang Jie Shi Haofei Hong Hongrui Xu Ling Li Guochao Liao Zhimeng Wu Zhifang Zhou 
supported by the National Natural Science Foundation of China(Nos.21907038 and 32000904);Natural Science Foundation of Jiangsu Province(No.BK20200601);National Postdoctoral Program for Innovative Talents of China(No.BX20200153);China Postdoctoral Science Foundation(Nos.2018M632227 and2021M691293);the Social Development Key Project of Jiangsu Province(No.BE2019632);the Health and Family Planning Commission of Wuxi,China(No.Z202005);Suzhou People’s Livelihood Science and Technology Project,China(No.SYS2018100);supported by the 111 Project(No.111-2-06)。
We construct MUC1 vaccines usingβ-cyclodextrin grafted chitosan(CS-g-CD)as carrier via host-guest interaction.These vaccines based on non-covalent assembling can provoke robust immune responses,including high level o...
关键词:β-Cyclodextrin grafted chitosan MUC1 antigen Cancer vaccine Host-guest interaction Vaccine carrier Tn antigen 
Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal被引量:3
《Systems Microbiology and Biomanufacturing》2022年第3期580-588,共9页Qiang Wang Weilai Fu Ruiqi Lu Chunli Pan Ganfeng Yi Xian Zhang Zhiming Rao 
supported by the Foundation of Fujian Key Laboratory of Functional Aquafeed and Culture Environment Control(No.FACE20200003);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,Top-notch Academic Programs Project of Jiangsu Higher Education Institutions,1.111 Project(111-2-06);Jiangsu province"Collaborative Innovation Center for Advanced Industrial Fermentation"industry development program.
An efficient ammonia nitrogen degrading bacterial strain was isolated from a fish and shrimp pond and identified as Bacillus subtilis(Ab03).Firstly,the strain was continuously domesticated in ammonium solution to impr...
关键词:Bacillus subtilis Ammonia nitrogen Nitrogen balance Simultaneous conversion 
Determination of ribose and phosphorus contents in Haemophilus influenzae type b capsular polysaccharide by a quantitative NMR method using a single internal standard被引量:2
《Chinese Journal of Natural Medicines》2022年第8期633-640,共8页QIN Chun-Jun HU Jing TONG Wei ZHANG Teng-Teng TIAN Guang-Zong ZoU Xiao-Peng LIU Jian-Kai YIN Jian 
supported by the National Natural Science Foundation of China(Nos.22077052,21877052,21907039 and 22107037);the China Postdoctoral Science Foundation(Nos.2020M681487 and 2021M691279);the National Key R&D Program of China(No.2020YFA0908304);the Natural Science Foundation of Jiangsu Province(Nos.BK20180030 and BK20190575);the National First-class Discipline Program of Light Industry Technology and Engineering(No.LITE2018-14);the 111 Project(No.111-2-06);the Open Project of Key Laboratory of Carbohydrate Chemistry and Biotechnology(Jiangnan University),Ministry of Education(No.KLCCB-KF202005)。
The ribose and phosphorus contents in Haemophilus influenzae type b(Hib)capsular polysaccharide(CPS)are two important chemical indexes for the development and quality control of Hib conjugate vaccine.A quantitative ^(...
关键词:Haemophilus influenzae type b Capsular polysaccharide Quantitative^(1)H-NMR Quantitative^(31)P-NMR Ribose content Phosphorus content 
Chemical approaches towards installation of rare functional groups in bacterial surface glycans被引量:1
《Chinese Journal of Natural Medicines》2022年第6期401-420,共20页QIN Chun-Jun DING Mei-Ru TIAN Guang-Zong ZOU Xiao-Peng FU Jun-Jie HU Jing YIN Jian 
This work was supported by the National Natural Science Foundation of China(Nos.22077052,21877052,21907039,22107037);China Postdoctoral Science Foundation(2020M681487,2021M691279);the National Key R&D Program of China(2020YFA0908304);the Natural Science Foundation of Jiangsu Province(BK20180030,BK20190575);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-14);the 111 Project(111-2-06);the Open Project of Key Laboratory of Carbohydrate Chemistry and Biotechnology(Jiangnan University),Ministry of Education(KLCCB-KF202005).
Bacterial surface glycans perform a diverse and important set of biological roles,and have been widely used in the treatment of bacterial infectious diseases.The majority of bacterial surface glycans are decorated wit...
关键词:Bacterial surface glycan Chemical synthesis Amido group Acetamidino group Carboxamido group Pyruvyl ketal 
Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol被引量:2
《Synthetic and Systems Biotechnology》2022年第2期765-774,共10页Xinyu Yu Xiuxia Liu Xiong Gao Xunxun Luo Yankun Yang Ye Li Chunli Liu Chong Zhang Zhonghu Bai 
This work received funding from the National Natural Science Foundation of China(No.21878124,22078128,and 21938004);the Fundamental Research Funds for the Central Universities(No.JUSRP221032);the 111 Project(No.111-2-06);the national first-class discipline program of Light Industry Technology and Engineering(LITE2018-24).
Corynebacterium glutamicum represents an emerging recombinant protein expression factory due to its ideal features for protein secretion,but its applicability is harmed by the lack of an autoinduction system with tigh...
关键词:Corynebacterium glutamicum ETHANOL Transcriptional engineering Secretory protein overexpression NEO-2/15 
Met196突变提高Brucella melitensis 7α-羟基类固醇脱氢酶的催化效率和稳定性被引量:1
《微生物学报》2022年第5期1769-1783,共15页柳志永 张荣珍 徐岩 
国家重点研发计划(2018YFA0900302);国家自然科学基金(31970045);江苏省研究生科研与实践创新计划(1012050205205974);国家轻工技术与工程一流学科(LITE2018-12);高等学校学科创新引智计划(111-2-06)。
【目的】通过计算机辅助设计,理性提高羊布鲁氏菌7α-羟基类固醇脱氢酶的催化效率和稳定性,实现酶的高效稳定催化合成。【方法】通过同源建模、分子对接和蛋白-配体相互作用分析,理性设计关键位点的定向突变。结合酶学性质测定、酶促反...
关键词:羊布鲁氏菌 7α-羟基类固醇脱氢酶 理性设计 催化效率 稳定性 分子动力学模拟 
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