Supported by the National High-Tech Research and Development (863) Program of China (No. 2003AA603430)
Alkaline phosphatase from Pinctada fucata was inactivated by o-phthalaldehyde (OPA). The inactivation followed pseudo first-order kinetics with a second rate constant of 0.167 (mmol/L)^-1·min^-1 at pH 7.5 and 25...