Supported by the National High-Tech Research and Development (863) Program of China (No. 2003AA603430)
Alkaline phosphatase from Pinctada fucata was inactivated by o-phthalaldehyde (OPA). The inactivation followed pseudo first-order kinetics with a second rate constant of 0.167 (mmol/L)^-1·min^-1 at pH 7.5 and 25...
Treatment of lactate dehydrogenase (LDH) with o phthalaldehyde resulted in a time dependent loss of enzyme activity. The inactivation followed pseudo first order kinetics over a wide range of the inhibitor. The ...