DEGLYCOSYLATION

作品数：4被引量：3H指数：1
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相关领域：生物学更多>>
相关作者：贺明吉永华何慧琼杨宏天徐清更多>>
相关机构：上海大学中国科学院上海生命科学研究院更多>>
相关期刊：《Neuroscience Bulletin》《Acta Biochimica et Biophysica Sinica》《hLife》《BIOCELL》更多>>
相关基金：国家自然科学基金更多>>
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Targeted protein editing technique in living mammalian cells by peptide-fused PNGase
《hLife》2024年第11期576-591,共16页Min Wu Guijie Bai Ziyi Zhang Haixia Xiao Wenliang Sun Chaoguang Tian 
supported by funding from the Key Project of the Ministry of Science and Technology of China(2018YFA0900500).
Various precise gene editing techniques at the DNA/RNA level,driven by clustered regularly interspaced short palindrome repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology,have gained significant prominence.Ye...
关键词：N-glycosylated proteins peptide:N-glycanase(PNGase) protein-targeting peptides DEGLYCOSYLATION protein sequence editing protein degradation 
Deglycosylation altered the gating properties of rNav1.3:glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel
《Neuroscience Bulletin》2008年第5期283-287,共5页徐清 程慧雯 何慧琼 刘志睿 贺明 杨宏天 周智磊 吉永华 
the National Basic Research Development Program of China (No. 2006CB500801).
Objective To examine the effect of deglycosylation on gating properties of rNav1.3. Methods rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was ...
关键词：rNav1.3 voltage-gated sodium channel GLYCOSYLATION two-electrode voltage clamp Xenopus oocyte 
Influence of Substrate Conformation on the Deglycosylation of Ribonuclease B by Recombinant Yeast Peptide:N-glycanase
《Acta Biochimica et Biophysica Sinica》2007年第1期8-14,共7页Shengjun WANG Peng George WANG and Qingsheng QI State Key Laboratory of Microbial Technology,Life Science Schooll,Shandong University,Jinan 250100,China 
This work was supported by a grant from National Natural Science Foundation of China(No. 30470399)
Peptide：N-glycanase has been thought to be responsible for proteasome-dependent degradation of misfolded glycoproteins translocated from the endoplasmic reticulum （ER） to the cytosol. Therefore, the enzyme was supp...
关键词：glycanase ribonuclease B GLYCOPROTEIN DEGLYCOSYLATION circular dichroism 
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