BRUCELLA

作品数：70被引量：185H指数：6
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小鼠IL-1β原核表达及其与在布鲁氏杆菌侵染巨噬细胞中差异表达的初步研究
《湖北农业科学》2022年第6期112-116,121,共6页杨馨 邹德颖 张凯 常江 常恒祯 战俊澎 郭珣 刘益辛 程梦妍 韩成 胡盼 卢士英 李岩松 柳增善 任洪林 
吉林省科技发展计划资助项目(20200402059NC);中央高校基本科研业务费专项资金资助项目(45121031B007)。
为研究IL-1β在布鲁氏杆菌(Brucella)侵染小鼠巨噬细胞过程中的表达情况,首先构建重组小鼠IL-1β原核稳定表达系统,免疫新西兰兔获得多克隆抗体,通过ELISA检测血清效价,并通过Western blot分析其活性,再以感染复数(multiplicity of infe...
关键词：布鲁氏杆菌(Brucella) 原核表达 多克隆抗体 IL-1Β MYD88 
Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China被引量：5
《Infectious Diseases of Poverty》2021年第2期64-69,共6页Zhi-Jun Zhao Ji-Quan Li Li Ma Hong-Mei Xue Xu-Xin Yang Yuan-Bo Zhao Yu-Min Qin Xiao-Wen Yang Dong-Ri Piao Hong-Yan Zhao Guo-Zhong Tian Qiang LiJian-Ling Wang Guang Tian Hai Jiang Li-Qing Xu 
supported by National Key R&D Program of China(Grant No.2020YFA0907101);Major Infectious Diseases such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects(2018ZX10201002);the National Natural Scientific Foundation of China(81860588).
Background:The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly,with confirmed cases distributed across 31 counties.However,the epidemiology of brucellosis transmission has not ...
关键词：Brucella m elitensis Multiple-locus variable-number tandem repeats analysis Whole-genome sequencing Single-nucleotide polymorphism 
Simultaneous detection and differentiates of Brucella abortus and Brucella melitensis by combinatorial PCR被引量：5
《Asian Pacific Journal of Tropical Medicine》2012年第1期24-28,共5页Reza Mirnejad Reza Hosseini Doust Reza Kachuei Seied Mojtaba Mortazavi Mehdi Khoobdel Ali Ahamadi 
supported by Molecular Biology Research Center,Baqiyatallah University of Medical Sciences,with grant number BMSU/MBRC- 89-009
Objective:To evaluate simultaneous detection and differentiates of Brucella abortus(B.abortus) and Brucella melitensis(B.melitensis) through the combinatorial POR method.Methods:This study was designed using three pri...
关键词：BRUCELLA ABORTUS BRUCELLA MELITENSIS Combinatorial PCR 
Immunochemical characterization of antigens of Brucella canis and their use in seroprevalence study of canine brucellosis
《Asian Pacific Journal of Tropical Medicine》2011年第11期857-861,共5页Sharma Barkha Sinha Dharmendra Kumar Singh Dhirendra Kumar 
The financial support provided to the first author in the form of Junior Fellowship by ICAR,New Delhi is duly acknowledged
Objective:To explore immunochemical characterization of antigens of Brucella canis(B. canis),and the use in seroprevalence study of canine brucellosis.Methods:External hot phosphate buffer saline extract(HPBSE) and in...
关键词：BRUCELLA CANIS SDS-PAGE Western BLOTTING AGAR gel IMMUNODIFFUSION test Dot-ELISA I-ELISA 
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