This project was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences.
A membrane-bound protein was purified from rat liver mitochondria.After being di-gested with V8 protease,two peptides containing identical 14 amino acid residue sequences were obtained.Using the 14 amino acid peptide ...
Project supported by China Postdoctoral Science Foundation and the National Natural Science Foundation of China.Abbteviation: CuZn-SOD,copper zinc superoxide dismutase; ET, electron-transfer;GuHCl,guanidine hydrochloride;MES, 2-morpholinoethane sulfonic
The electron-transfer (ET) reaction between Fe(CN)64- and copper zinc superoxide dismutase (CuZn-SOD) occurs at the active site of the enzyme. The ET parameters which are sensitive to the denaturation have been used t...