We thank Drs Bo Zhang, Hui Yang and Filippo Del Bene for comments on the manuscript and Dr Bo Zhang for providing zCas9. This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02040003), the National Basic Research Program of China (973 Program; 2011CBA00400, 2012CB945101), the National Outstanding Young Scientist Program of the National Natural Science Foundation of China (31325011), Shanghai Subject Chief Scientist Program of the Science and Technology Commission of Shanghai (14XD1404100), and the Postdoctoral Research Program of Shanghai Institutes for Biological Sciences (2014KIP307).
Dear Editor, Animals carrying exogenous genes integrated at specific genomic loci are versatile tools for biological research [1]. Zebrafish (Danio rerio), an emerging vertebrate animal model, is widely used in stu...