supported by National Key Basic Research Special Foundation of China (No.G2000018601);the National Natural Science Foundation of China (No.30370239);Chinese Ministry of Education Key Project (No.03046).
Endophyte-infected(EI)seeds of Lolium perenne L.were used to attain endophyte-free(EF)population by heating the seeds at 43℃for 15 min and then 57℃for 25 min.Relative water content(RWC),chlorophyll,soluble sugar and...