COPY_NUMBER

作品数:56被引量:88H指数:5
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相关机构:中国科学院遗传与发育生物学研究所广州医科大学复旦大学南京大学更多>>
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  • 期刊=Synthetic and Systems Biotechnologyx
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Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch
《Synthetic and Systems Biotechnology》2024年第3期577-585,共9页Haoyu Guo Rongzhen Tian Yaokang Wu Xueqin Lv Jianghua Li Long Liu Guocheng Du Jian Chen Yanfeng Liu 
supported by the National Key Research and Development Program of China(2020YFA0908300);the National Natural Science Foundation of China(32222069,32172349);the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(32021005);the Natural Science Foundation of Jiangsu Province(BK20202002).
Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic biology.In comparison to pla...
关键词:Bacillus subtilis Stabilized gene amplification system Homologous recombination RECA 
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica被引量:4
《Synthetic and Systems Biotechnology》2023年第2期331-338,共8页Man Xu Wenliang Xie Zhen Luo Chun-Xiu Li Qiang Hua Jian-He Xu 
supported by the National Key Research and Development Program of China(2019YFA0905000);the National Natural Science Foundation of China(21871085 and 31971380);the Fundamental Research Funds for the Central Universities(222201714026).
Taxadiene is an important precursor for the biosynthesis of highly effective anticancer drug paclitaxel,but its microbial biosynthesis yield is very low.In this study,we employed Yarrowia lipolytica as a microbial hos...
关键词:Taxadiene Yarrowia lipolytica “Push–pull”ostrategy Multi-copy integration Fed-batch fermentation 
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