supported partly by the National Natural Science Foundation of China(Grant Nos.30025003&30070034);the Hundreds-Talent Program and Knowledge Innovation Program(Grant No.kscx2-1-02,kscx2-SW-301-09)of the Chinese Academy of Sciences.
This paper describes a rapid method of constructing homologous recombinant baculovirus in E. coli with PCR-amplified fragments. By using this method, the traditional steps of constructing transfer vector are omitted. ...