hsa_circ_0087378在食管鳞状细胞癌组织中的表达及其对患者预后的影响  

Expression of hsa_circ_0087378 in esophageal squamous cell carcinoma tissues and its effect of prognosis of patients

作　　者：朱永刚[1,2] 王明博[2] 孟令娇 单保恩[1] ZHU Yonggang;WANG Mingbo;MENG Lingjiao;SHAN Baoen(Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China;Thoracic Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)

机构地区：[1]河北医科大学第四医院科研中心,河北石家庄050011 [2]河北医科大学第四医院胸外科,河北石家庄050011

出　　处：《中国肿瘤生物治疗杂志》2022年第4期327-331,共5页Chinese Journal of Cancer Biotherapy

基　　金：河北省卫健委科研基金资助项目(No.20210074)

摘　　要：目的:探讨hsa_circ_0087378在食管鳞状细胞癌(ESCC)组织中的表达及其对ESCC患者临床病理指标及预后的影响。方法:通过GEO数据库筛选ESCC组织及相应癌旁组织中差异表达的circRNA,采用qPCR法在10例ESCC组织及相应癌旁组织中进行验证。选取河北医科大学第四医院2015年10月至2016年4月收治的90例ESCC患者癌组织及配对癌旁组织标本,通过FISH法检测hsa_circ_0087378的表达,分析其表达与ESCC患者临床病理指标及预后的关系。结果:GEO数据库及qPCR分析结果显示,hsa_circ_0087378在ESCC组织中表达最明显。FISH结果显示,90例癌旁组织中21例hsa_circ_0087378呈高表达,高表达率为23.3%;90例ESCC组织中有65例hsa_circ_0087378呈高表达,高表达率为72.2%,癌组织中表达明显高于癌旁组织(P<0.05)。hsa_circ_0087378在ESCC组织中的高表达与患者的临床分期和淋巴结转移有关联(P<0.05),hsa_circ_0087378高表达组ESCC患者的5年总生存率明显低于低表达组患者(P<0.05)。结论:hsa_circ_0087378在ESCC组织中呈高表达,且与患者的临床分期与淋巴结转移和预后不良有关,其可能是ESCC的治疗靶点。Objective:To investigate the expression of hsa_circ_0087378 in esophageal squamous cell carcinoma(ESCC)tissues,and its effect on the clinicopathological characteristics and prognosis of ESCC patients.Methods:The differentially expressed circRNAs between ESCC tissues and corresponding para-cancerous tissues were screened by GEO database,which were then validated in 10 pairs of ESCC tissues and corresponding para-cancerous tissues by qPCR method.90 pairs of cancer tissues and corresponding paracancerous tissues resected from ESCC patients who admitted to the Fourth Hospital of Hebei Medical University from October 2015 to April 2016 were collected to detect the expression of hsa_circ_0087378 by FISH method,and the relationship between the expression of hsa_circ_0087378 and the clinicopathological indicators and prognosis of ESCC patients were also analyzed.Results:GEO database and q PCR analysis results showed that hsa_circ_0087378 was significantly up-regulated in ESCC tissues.FISH results showed that hsa_circ_0087378 were highly expressed in 21(23.3%)of 90 cases of para-cancerous tissues,but in 65(72.2%)of 90 cases of ESCC tissues.The hsa_circ_0087378 over-expression rate in ESCC tissues was significantly higher than that in para-cancerous tissues(P<0.05).The high expression of hsa_circ_0087378 in ESCC tissues was related to the clinical stage and lymph node metastasis of ESCC patients(P<0.05).The 5-year overall survival rate of patients with high hsa_circ_0087378 expression was significantly lower than that in patients with low circ_0087378 expression(P<0.05).Conclusion:hsa_circ_0087378 is highly expressed in ESCC tissues and positively correlated with the clinical stage,lymph node metastasis,and poor prognosis of patients,suggesting that hsa_circ_0087378 can be used as a potential therapeutic target for ESCC.

关 键 词：食管鳞状细胞癌 环状RNA hsa_circ_0087378 预后 

分 类 号：R735.1[医药卫生—肿瘤]