金花茶化学成分及抗炎抗氧化活性研究  

Studies on the chemical constituents of Camellia nitidissima Chi leaves and their anti-inflammatory,anti-oxidant activity

作　　者：温静[1] 梁伟[1] 王欣晨 李彤彤 刘萍 白鑫宇 李文兰[1] 阎新佳 WEN Jing;LIANG Wei;WANG Xin-chen;LI Tong-tong;LIU Ping;BAI Xin-yu;LI Wen-lan;YAN Xin-jia(College of Pharmacy,Harbin University of Commerce,Harbin 150076,China;College of Vocational Technology Education,Harbin University of Commerce,Harbin 150076,China;Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [2]哈尔滨商业大学职业教育学院,黑龙江哈尔滨150076 [3]遵义医科大学教育部基础药理学重点实验室,贵州遵义563000

出　　处：《中国药物化学杂志》2020年第8期487-492,共6页Chinese Journal of Medicinal Chemistry

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2017201);2019年度哈尔滨商业大学“青年创新人才”支持计划(2019CX11);黑龙江省教育科学“十三五”规划2017年度重点课题(GZB1317067);黑龙江省教育厅青年创新人才项目(17XN057);哈尔滨商业大学研究生科研创新项目(YJSCX2020-633HSD)

摘　　要：目的对金花茶(Camellia nitidissima Chi)的化学成分及其抗炎、抗氧化活性进行研究。方法通过聚酰胺、硅胶、ODS和pre-HPLC等色谱方法对金花茶叶的体积分数95%的乙醇提取物进行分离纯化,通过理化性质和波谱学手段鉴定化合物的结构;考察金花茶叶的不同萃取部位对脂多糖刺激的小鼠单核巨噬细胞(RAW 264.7)NO释放量的影响;采用DPPH法检测金花茶不同部位的抗氧化活性。结果从金花茶叶中分离得到7个化合物,分别鉴定为泽兰酸(1)、槲皮素(2)、正三十三烷(3)、β-香树脂醇(4)、邻苯二甲酸二丁酯(5)、1,6-己内酰胺(6)和芦丁(7)。金花茶叶的不同萃取部位均可以明显抑制RAW 264.7细胞NO的释放,同时均有一定程度的抗氧化活性;金花茶植物不同部位的抗氧化效果顺序为花蕾>花蕊>花瓣>叶子。结论化合物1和3为首次从山茶属植物中分离得到,化合物4~6为首次从金花茶中分离得到;金花茶具有显著抗炎及抗氧化作用,该研究为金花茶作为功能食品的开发提供技术参考和理论依据。Seven compounds were isolated from Camellia nitidissima Chi leaves and were identified as eupatoric acid(1),quercetin(2),tritriacontane(3),β-amyrin(4),dibutyl phthalate(5),1,6-caprolactam(6)and rutin(7).Their structures were identified by physicochemical properties and spectral data.Compounds 1 and 3 were isolated from the genus for the first time,and compounds 4-6 were first found from this plant.Different extracts of C.nitidissima had very significant inhibitory effects on inflammatory mediators in RAW 264.7 cells.The n-butanol extract of C.nitidissima exhibited significant anti-oxidant activity.Moreover,the anti-oxidant activity of different parts from C.nitidissima was in the decreasing order of extract from flower bud>stamen>petal>leaf.Therefore,C.nitidissima has obvious anti-inflammatory and anti-oxidant effects and this study provides a scientific basis to develop C.nitidissima as a natural anti-oxidant and anti-inflammatory resource.

关 键 词：金花茶 化学成分 分离鉴定 抗炎活性 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]