虾青素对支气管哮喘大鼠气道炎症及气道重构的影响  

The effects of astaxanthin on the airway inflammation and remodeling in the asthmatic rats

作　　者：李萌 张令轩 宋歌 高福生[2] LI Meng;ZHANG Lingxuan;SONG Ge;GAO Fusheng(School of Clinical Medicine,Weifang Medical University,Weifang,Shandong 261000,P.R.China;Department of Respiratory and Critical Care Medicine,the Affiliated Hospital of Weifang Medical College,Weifang,Shandong 261000,P.R.China)

机构地区：[1]潍坊医学院临床医学院,山东潍坊261000 [2]潍坊医学院附属医院呼吸与危重症医学科,山东潍坊261000

出　　处：《中国呼吸与危重监护杂志》2024年第8期575-582,共8页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的观察虾青素(astaxanthin,AST)对支气管哮喘大鼠气道炎症及气道重构的影响。方法将50只雄性Wistar大鼠按随机数字表法分为5组(n=10):生理盐水致敏+生理盐水激发组(对照组)、支气管哮喘组(哮喘组)、支气管哮喘+虾青素5 mg/kg灌胃处理组(AST 5 mg/kg组)、10 mg/kg灌胃处理组(AST 10 mg/kg组)、50 mg/kg灌胃处理组(AST 50 mg/kg组)。酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法测定支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)、白介素5(interleukin-5,IL-5)、白介素13(interleukin-13,IL-13)、干扰素-γ(interferon-γ,IFN-γ)、转化生长因子β(tansforming growth factor-β,TGF-β)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)及血清总IgE水平;肺组织切片苏木素-伊红(hematoxylin-eosin,HE)染色观察气道炎症细胞浸润和上皮细胞脱落程度;过碘酸雪夫染色(periodic acid schiff,PAS)观察气道上皮杯状细胞增生程度;Masson染色观察气道上皮下胶原纤维沉积的程度;逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)测肺组织中黏蛋白5AC(mucin 5A and C,MUC5AC)信使核糖核酸(messenger ribonucleic acid,mRNA)表达;免疫组织化学染色测定大鼠气道上皮MUC5AC蛋白表达。结果哮喘组大鼠BALF中IL-5、IL-13、TGF-β、MDA、血清总IgE分别为[(36.73±2.29)、(53.99±2.70)、(60.89±2.54)ng/mL、(18.65±0.76)umol/L、(54.50±2.91)ng/mL]、炎症细胞(46.24±4.26)、嗜酸性粒细胞(2.09±0.13)、脱落上皮/气道上皮[(6.09±0.45)%]、杯状细胞/上皮细胞面积比值[(13.65±1.90)%]、胶原纤维/基底膜下20μm区域面积[(17.58±2.14)%]、肺组织MUC5AC mRNA、肺组织MUC5AC蛋白表达IOD值(187±12)均高于对照组(P<0.01或P<0.001),IFN-γ[(26.38±1.70)ng/mL]、SOD[(16.37±1.22)U/L]低于对照组(P<0.001);AST处理组IL-5、IL-13、总IgE、TGF-β、MDA水平、气道上皮炎症细胞浸润和上皮细胞损伤脱落程度、气道上皮Objective To observe the effects of astaxanthin(AST)on the airway inflammation and remodeling in the asthmatic rats.Methods Fifty male Wistar rats were randomly divided into five groups(n=10 for each group):saline-sensitized and-saline-challenged group(the control group),bronchial asthma group(the asthma group),bronchial asthma+astaxanthin 5 mg/kg gavage treatment group(the AST 5 mg/kg group),bronchial asthma+10 mg/kg gavage treatment group(the AST 10 mg/kg group),and bronchial asthma+50 mg/kg gavage treatment group(the AST 50 mg/kg group).The level of interleukin-5(IL-5),interleukin-13(IL-13),interferon-γ(IFN-γ),tansforming growth factor-β(TGF-β),malondialdehyde(MDA)and superoxide dismutase(SOD)in the bronchoalveolar lavage fluid(BALF)and the total IgE level in the serum were measured using enzyme linked immunosorbent assay(ELISA).The infiltration of airway inflammatory cells and the degree of airway epithelial cells detachment,the extent of goblet cell hyperplasia and the severity of subepithelial collagen deposition were evaluated on the hematoxylin eosin(HE),periodic acid Schiff(PAS)and Masson trichrome stained lung sections.reverse transcription-polymerase chain reaction(RT-PCR)was used to measure the expression of mucin 5A and C(MUC5AC)messenger ribonucleic acid(mRNA)in lung tissue;Immunohistochemical staining was used to determine the expression of MUC5AC protein in the rat airway epithelium.Results The level of IL-5,IL-13,TGF-β,MDA and the total IgE in the serum respectively[(36.73±2.29),(53.99±2.70),(60.89±2.54)ng/mL,(18.65±0.76)umol/L,(54.50±2.91)ng/mL],the extent of inflammatory cells infiltration(46.24±4.26),the extent of eosinophils infiltration(2.09±0.13),the extent of epithelial cells detachment[(6.09±0.45)%],the extent of goblet cell hyperplasia[(13.65±1.90)%],the extent of subepithelial collagen deposition[(17.58±2.14)%],the MUC5AC mRNA expression level,and the lung tissue MUC5AC protein expression IOD value(187±12)in the asthma group were all higher than those in the control grou

关 键 词：虾青素 哮喘 气道炎症 气道重构 黏蛋白 

分 类 号：R562.25[医药卫生—呼吸系统]