人脐带动脉旁、静脉旁与华通氏胶间充质干细胞的血管生成能力比较及移植安全性评价  

Comparison of angiogenesis ability and transplantation safety among mesenchymal stem cells derived from umbilical artery,umbilical vein and Wharton’s jelly

作　　者：杨嫣君 余飞[3] 丁利军[4,5] 盛小强 孙海翔[1,4] YANG Yanjun;YU Fei;DING Lijun;SHENG Xiaoqiang;SUN Haixiang(Center for Reproductive Medicine,Drum Tower Clinic Medical College of Nanjing Medical University,Nanjing 210008,China;Department of Gynecology,Changzhou First People's Hospital,Changzhou 213000;Experimental Animal Center,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008;Center for Reproductive Medicine,Department of Obstetrics and Gynecology,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008;Clinical Center for Stem Cell Research,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008)

机构地区：[1]南京医科大学鼓楼临床医学院生殖医学中心,南京210008 [2]常州市第一人民医院产科,江苏常州213000 [3]南京大学医学院附属鼓楼医院动物实验中心,南京210008 [4]南京大学医学院附属鼓楼医院生殖医学中心,南京210008 [5]南京大学医学院附属鼓楼医院临床干细胞研究中心,南京210008

出　　处：《中国比较医学杂志》2020年第2期43-53,共11页Chinese Journal of Comparative Medicine

基　　金：国家自然科学青年基金项目(81801518);江苏省科技计划(BE2018602).

摘　　要：目的探讨人脐带动脉旁、静脉旁与华通氏胶间充质干细胞的血管生成能力及其生物安全性。方法分离培养人脐带动脉旁、静脉旁与华通氏胶间充质干细胞,流式细胞仪鉴定表面标记物,体外分化实验检测其多向分化潜能,免疫荧光法检测血管旁细胞标记物,体外成环检测三种干细胞的血管生成能力差异;三种干细胞移植入NOD-SCID小鼠检测其生物安全性。结果三种干细胞表达间充质干细胞标记物,具有多向分化潜能,脐带动脉旁干细胞表达丰度更高的血管旁细胞标记物,且体外血管生成能力更强;三种干细胞移植后3月,小鼠主要器官无明显形态学损伤与异常。结论人脐带动脉旁干细胞血管生成能力更强;三种干细胞移植NOD-SCID小鼠无显著成瘤性,安全可行。Objective To investigate and compare the angiogenesis ability and transplantation safety of mesenchymal stem cells derived from umbilical artery,umbilical vein and Wharton’s jelly.Methods We isolated and cultured human umbilical cord artery perivascular stem cells(UCA-PSCs),umbilical cord vein perivascular stem cells(UCV-PSCs)and Wharton’s jelly mesenchymal stem cells(WJ-MSCs).Flow cytometry was used to analyze the expression of cell surface markers at passage 3.The multipotency of cells was analyzed by adipogenic,osteogenic and neural-like differentiation assays.Immunofluorescence was used to detect the perivascular stem cell markers.Angiogenesis ability was analyzed by tube formation assay.Stem cells were transplanted into nonobese diabetic/severe combined immunodeficiency(NOD-SCID)mice to test the biological safety.Results UCA-PSCs,UCV-PSCs and WJ-MSCs expressed mesenchymal stem cell markers that showed multi-lineage differentiation potential.UCA-PSCs expressed perivascular stem cell markers and had stronger angiogenesis ability in vitro than UCV-PSCs and WJ-MSCs.At 3 months after cell transplantation,hematoxylin and eosin staining showed no obvious morphological damage or abnormality in the organs.Conclusions UCA-PSCs had stronger angiogenesis ability than UCV-PSCs and WJ-MSCs.The transplantation of three kinds of stem cells into NOD-SCID mice result ed in no tumor formation,and appeared to be safe and feasible.

关 键 词：脐带动脉旁干细胞 血管生成 生物安全性 

分 类 号：R-33[医药卫生]