免疫组织化学技术在确定非黑素瘤性皮肤癌手术切缘中的应用进展  

Application of Immunohistochemistry in Determining the Surgical Margin of Non-Melanoma Skin Cancer

作　　者：黄淑黛 王大光[1] HUANG Shudai;WANG Daguang(Department of Dermatology and Venereology,the First Affiliated Hospital with Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院皮肤科,江苏南京210029

出　　处：《中国皮肤性病学杂志》2022年第12期1442-1445,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81000703,81472896);江苏省自然科学基金(BK2009437);江苏省六大人才高峰(2015-WSW-026)

摘　　要：Mohs显微描记手术目前已成为治疗非黑素瘤性皮肤癌的标准手术术式。在大量炎性细胞浸润、周围神经侵犯或解剖结构特殊时,术中常规HE染色可能无法精准判断手术切缘,从而导致漏切和癌症复发。联合免疫组织化学技术检测特异性表达的标志物有助于进一步明确切缘性质。荧光引导手术可在术中实时评估肿瘤边缘,并指导手术切除。本文旨在对免疫组织化学技术确定基底细胞癌、皮肤鳞状细胞癌、乳房外Paget病、隆突性纤维肉瘤和皮脂腺癌手术切缘中的应用进行综述。Mohs micrographic surgery is currently the standard treatment of non-melanoma skin cancers. Since HE(hematoxylin-eosin staining) staining during the Mohs micrographic surgery sometimes fails to determine the positive expressions of tumor cells accurately, because of abundant inflammatory cells infiltration, peripheral nerve invasion or special anatomical structure, pathological errors may inevitably lead to less resection and relapse of non-melanoma skin cancers. The combination of immunohistochemical techniques will help to further clarify the nature of the tumor margin.Fluorescence-guided surgery can evaluate tumor margin during the operation timely and guide the surgical resection accurately. This study aims to review the application of immunohistochemistry in determining tumor margins of basal cell carcinoma, cutaneous squamous cell carcinoma, extramammary Paget′s disease, dermatofibrosarcoma protuberans and sebaceous carcinoma.

关 键 词：非黑素瘤性皮肤癌 免疫组织化学技术 HE染色 Mohs显微描记手术 荧光引导手术 

分 类 号：R739.5[医药卫生—肿瘤]