Cryo-EM structure of glycoprotein C from Crimean-Congo hemorrhagic fever virus  

作　　者：Na Li Guibo Rao Zhiqiang Li Jiayi Yin Tingting Chong Kexing Tian Yan Fu Sheng Cao 

机构地区：[1]CAS Key Laboratory of Special Pathogens,Center for Biosafety Mega-Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China [2]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Virologica Sinica》2022年第1期127-137,共11页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31570161)

摘　　要：Crimean-Congo hemorrhagic fever virus(CCHFV)is a causative agent of serious hemorrhagic diseases in humans with high mortality rates.CCHFV glycoprotein Gc plays critical roles in mediating virus-host membrane fusion and has been studied extensively as an immunogen.However,the molecular mechanisms involved in membrane fusion and Gc-specific antibody-antigen interactions remain unresolved largely because structural information of this glycoprotein is missing.We designed a trimeric protein including most of the ectodomain region of Gc from the prototype CCHFV strain,Ib Ar10200,which enabled the cryo-electron microscopy structure to be solved at a resolution of 2.8Å.The structure confirms that CCHFV Gc is a class Ⅱ fusion protein.Unexpectedly,structural comparisons with other solved Gc trimers in the postfusion conformation revealed that CCHFV Gc adopted hybrid architectural features of the fusion loops from hantaviruses and domain Ⅲ from phenuiviruses,suggesting a complex evolutionary pathway among these bunyaviruses.Antigenic sites on CCHFV Gc that protective neutralizing antibodies target were mapped onto the CCHFV Gc structure,providing valuable information that improved our understanding of potential neutralization mechanisms of various antibodies.

关 键 词：Crimean-Congo hemorrhagic Fever virus(CCHFV) Glycoprotein C Fusion protein BUNYAVIRUS 

分 类 号：R373.32[医药卫生—病原生物学]