A systematic strategy for screening therapeutic constituents of Schisandra chinensis(Turcz.) Baill infiltrated blood-brain barrier oriented in lesions using ethanol and water extracts:a novel perspective for exploring chemical material basis of herb medicines  

A systematic strategy for screening therapeutic constituents of Schisandra chinensis(Turcz.) Baill infiltrated blood-brain barrier oriented in lesions using ethanol and water extracts:a novel perspective for exploring chemical material basis of herb medici

作　　者：Yiwen Zhang Xinyan Lv Jiameng Qu Xin Zhang Mingyang Zhang Hao Gao Qian Zhang Ran Liu Huarong Xu Qing Li Kaishun Bi 

机构地区：[1]School of Pharmacy,Shenyang Pharmaceutical University Shenyang 110016,China [2]School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Acta Pharmaceutica Sinica B》2020年第3期557-568,共12页药学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(U1508220);Liaoning DistinguishedProfessor Project for Qing Li(2017,China);Shenyang Pharmaceutical University Innovative Research Team.

摘　　要：Schisandra chinensis,a widely used Chinese herbal medicine,was considered as central nervous system(CNS)drug for years.Both ethanol extracts(EES)and water extracts(WES)of it were applied clinically.Unfortunately,the difference of their efficacy and even effective material foundation of S.chinensis remains obscure.In this study,to explore the active constituents of S.chinensis,we compared pharmacodynamics and chemical profiles in vitrolin vivo of EES/WES for the first time using multiple chemical analysis,pharmacological and data processing approaches.It was proved that there was no significant difference in the anti-depressive effects between WES and EES.However,the contents of most components in vitro and in plasma were higher in EES than those in WES,which was unconvincing for their similar efficacy.Therefore,we further explored components of S.chinensis targeted onto brain and the results showed that 5 lignans were identified with definite absorptivity respectively both in EES and WES caused by the limitation of blood-brain barrier.Moreover,bioinformatic analysis predicted their anti-depressive action.Above all,the systematic strategy screened 5 brain-targeted effective substances of S.chinensis and it was suggested that exploring the components into nidi would promote the studies on herbs effective material basis.Schisandra chinensis,a widely used Chinese herbal medicine,was considered as central nervous system(CNS) drug for years.Both ethanol extracts(EES) and water extracts(WES) of it were applied clinically.Unfortunately,the difference of their efficacy and even effective material foundation of S.chinensis remains obscure.In this study,to explore the active constituents of S.chinensis,we compared pharmacodynamics and chemical profiles in vitrolin vivo of EES/WES for the first time using multiple chemical analysis,pharmacological and data processing approaches.It was proved that there was no significant difference in the anti-depressive effects between WES and EES.However,the contents of most components in vitro and in plasma were higher in EES than those in WES,which was unconvincing for their similar efficacy.Therefore,we further explored components of S.chinensis targeted onto brain and the results showed that 5 lignans were identified with definite absorptivity respectively both in EES and WES caused by the limitation of blood-brain barrier.Moreover,bioinformatic analysis predicted their anti-depressive action.Above all,the systematic strategy screened 5 brain-targeted effective substances of S.chinensis and it was suggested that exploring the components into nidi would promote the studies on herbs effective material basis.

关 键 词：SCHISANDRA chinensis ETHANOL EXTRACTS Water EXTRACTS PHARMACODYNAMICS CHEMICAL profiles Components into nidi Effective material basis Traditional Chinese medicine 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]