裸鼠肺癌原位模型的建立及肿瘤对裸鼠生理功能的影响  

Establishment of orthotopic lung cancer model in nude mice and effectsof tumor on physiological function of mude mice

作　　者：孔丽娟 郝智慧[1] 王成 赵明沂[1] KONG Lijuan;HAO Zhihui;WANG Cheng;ZHAO Mingyi(School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2019年第10期920-925,共6页Journal of Shenyang Pharmaceutical University

摘　　要：目的通过尾静脉注射肿瘤细胞的方法,建立一种肺癌原位移植瘤裸鼠模型,并进一步研究肺癌肿瘤生长对动物生理功能的影响。方法40只裸鼠经尾静脉注射接种人肺腺癌A549细胞,分别在第14、21、24、27、30天各解剖一组,肉眼观察肺部有无肿瘤生长及脑、肝、肾等脏器转移情况,并取肺脏、肝脏进行病理HE染色、镜检;采用全自动生化分析仪检测血清中丙氨酸氨基转氨酶(ALT)、天冬氨酸氨基转移酶(AST)、尿素氮(BUN)等生化指标;取材称重,计算脏体系数。结果第21天动物肺部开始出现肿瘤结节;第27天出现死亡。除第14天解剖的动物因时间尚早未成瘤外,其余动物均有肿瘤生长。病理镜检结果为腺癌。与对照组比较,移植瘤动物的血清ALT、AST、CREA各阶段均有所升高、ALB各阶段有所降低,第21、24天的肝脏、脾脏器系数明显升高。结论通过尾静脉注射低浓度肿瘤细胞成功建立了人肺癌原位移植瘤模型;裸鼠的生理和免疫功能与肿瘤生长有很大关系。Objective To establish a model of nude mice orthotopic lung cancer by the method of tail vein injection of tumor cells and to further study the effects of lung cancer growth on visceral system and blood biochemical indexes of nude mice organs.Methods 40 nude mice were inoculated with Human lung adenocarcinoma A549 cells of lung adenocarcinoma.On the 14,21,24,27 and 30 d,each group was dissected to observe the metastasis of brain,liver,kidney and other organs,and thelung and liver were taken for pathological HE staining and microscopic examination.Automatic biochemical analyzer was used to detect alanine aminotransferase(ALT),aspartate aminotransferase(AST),urea nitrogen(BUN)in serum.Organs are weighed and dirty systems are counted are counted.Results On the 21 st day after inoculation,tumor nodules appeared in the animal′s lungs,and on the 27 th day the animal began to die.With the exception of the animals dissected on the 14 th day,which were still too early to from a tumor,the final model composition rate was 100%and the mortality rate was 17%.The anatomical findings showed that the lung structure was completely destroyed and the tumor cells were infiltrated with diffuse infiltration.The pathological finding were adenocarcinoma.Compared with the control group,the serm ALT,AST and CREA of the transplanted tumor were increased in all stages,and the ALB stage decreased.The liver and spleen were significantly increased in the 21 and 24 d.Conclusion The model of human lung cancer in nude mice is successfully established by intravenous injection of low concentration tumor cells.The physiological and immunological characteristics of the nude mice models have a great relationship with tumor growth.

关 键 词：裸鼠 尾静脉注射 人肺腺癌A549细胞 肺癌移植模型 

分 类 号：R-332[医药卫生] R734.2