高效液相色谱法检测酵母产品中的还原型谷胱甘肽  

Determination of Reduced Glutathione in Yeast by High Performance Liquid Chromatography

作　　者：刘皓[1] 韦淑毅 吕春晖[1] LIU Hao;WEI Shuyi;LYU Chunhui(Department of Biological Engineering,Tianjin Modern Vocational Technology College,Tianjin 300350,China;Multidisciplinary(Beijing)Technology Services Limited Company,Beijing 100190,China)

机构地区：[1]天津现代职业技术学院生物工程学院,天津300350 [2]曼迪匹艾(北京)科技服务有限公司,北京100190

出　　处：《食品科技》2020年第1期338-342,350,共6页Food Science and Technology

摘　　要：还原型谷胱甘肽是重要的抗氧化剂,也是所有酵母产品共有的、最重要的活性物质之一。文章建立适合于各类酵母产品中还原型谷胱甘肽检测的高效液相色谱检测法,采用稀酸提取法提取样品中的谷胱甘肽,邻苯二甲醛柱前衍生,反相高效液相色谱法分离,荧光法检测。检出限为(S/N=3)6.7μg/L,定量限为(S/N=10)22.5μg/L。在5~100 mg/L范围内,待测物浓度与荧光信号值的线性关系良好(r=0.9993)。回收率在92.11%~101.35%,方法相对标准偏差<1.6%。市售富硒酵母及硒营养补充剂中还原型谷胱甘肽的含量为(0.120±0.000)^(1.315±0.027)mg/g。市售高活性面包酵母中还原型谷胱甘肽的含量为(9.805±0.516)mg/g。所建立的方法快速(可在1.5 h内完成全部检测过程)、经济、可靠,可用于各类酵母产品中还原型谷胱甘肽的定量检测。Reduced glutathione had been an important antioxidant and one of the most important active substances common to all yeast products.To establish a high performance liquid chromatography method for the detection of reduced glutathione in various yeast products,the glutathione in the sample has been extracted by dilute acid extraction,pre-column derivatization of phthalaldehyde,separation by high performance liquid chromatography,fluorescence detection.The detection limit(S/N=3)of glutathione was 6.7μg/L,the quantification limit(S/N=10)was 22.5μg/L.In the range of 5~100 mg/L,the linear relationship between the concentration of the analyte and the fluorescence signal value was good(r=0.9993).The recovery rate was 92.11%~101.35%,and the relative standard deviation of the method was<1.6%.The content of reduced glutathione in commercially available selenium-enriched yeast and selenium nutritional supplements was(0.120±0.000)^(1.315±0.027)mg/g.The content of reduced glutathione in commercially available high-activity baker’s yeast is(9.805±0.516)mg/g.The established method is fast(completed in 1.5 h),economical and reliable,and can be used for quantitative detection of reduced glutathione in various yeast products.

关 键 词：富硒酵母 谷胱甘肽 高效液相色谱 荧光检测 

分 类 号：O657.72[理学—分析化学] TS218[理学—化学]