高效液相色谱法测定破布木果水提物中迷迭香酸的含量  

Determination of rosmarinic acid in water extract of Cordia dichotoma fruits by high performance liquid chromatography

作　　者：哈丽娅·吐尔逊麦麦提 米热阿依·亚力昆 热娜古丽·艾则孜[3] 买吾拉尼江·依孜布拉 HALIYA Tu-Er-Xun-Mai-Mai-Ti;MIRAYI Ya-Li-Kun;RENAGULI Ai-Ze-Zi;MAIWULANIJIANG Yi-Zi-Bu-La(School of Medicine,Xinjiang Medical University,Urumqi 830011,China;Pharmacy College,Xinjiang Medical University,Urumqi 830011,China;The Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Central Laboratory,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学维医学院,乌鲁木齐830011 [2]新疆医科大学药理学院,乌鲁木齐830011 [3]新疆医科大学第四附属医院,乌鲁木齐830011 [4]新疆医科大学中心实验室,乌鲁木齐830011

出　　处：《食品安全质量检测学报》2020年第4期1215-1219,共5页Journal of Food Safety and Quality

基　　金：新疆维吾尔自治区高校科研计划自然科学青年项目(XJEDU2017S024)。

摘　　要：目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定破布木果水提物中迷迭香酸的含量。方法用高效液相色谱法,色谱条件:Kromasil C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-1.5%乙酸水(38:62),流速为1.0 mL/min,柱温25℃,检测波长为330 nm,检测破布木果水提物中迷迭香酸的含量。结果迷迭香酸峰面积与进样量在15.625~1000μg/mL范围内呈良好的线性关系(r=0.9996),平均回收率为98.68%,相对标准偏差为2.13%(n=6)。结论HPLC法具有操作简便、结果准确可靠的优势,可用于检测破布木果水提物中迷迭香酸含量。Objective To establish a method for determination of rosmarinic acid content in water extract of Cordia dichotoma fruits by high performance liquid chromatography(HPLC).Methods HPLC was used to determine the content of rosmarinic acid in water extract of Cordia dichotoma fruits.Chromatographic conditions were as follow:chromatographic column was Kromasil C18 column(4.6 mm×150 mm,5μm),mobile phase was methanol-1.5%acetic acid water(38:62),flow rate was 1.0 mL/min,column temperature was 25℃,detection wavelength was at 330 nm.Results The peak area of rosmarinic acid and the injection volume showed a good linear relationship(r=0.9996)in the range of 15.625-1000μg/mL,the average recovery was 98.68%,and the relative standard deviation was 2.13%(n=6).Conclusion This method is simple,accurate and reliable,and it is feasible for the determination of rosmarinic acid content in water extract of Cordia dichotoma fruits.

关 键 词：高效液相色谱法法 破布木果水提物 迷迭香酸含量 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]