电针内关、公孙穴对功能性消化不良大鼠海马GluR1、GluR2及内脏高敏性的影响  

Effects of Electroacupuncture at SP4 and PC6 on GluR1 and GluR2 in Hippocampus and Visceral Hypersensitivity in Functional Dyspepsia Rats

作　　者：覃思敏 谢云方 邢博文 展立芬 伍萍香 江钰 石海斌 刘未艾[1] Qin Simin;Xie Yunfang;Xing Bowen;Zhan Lifen;Wu Pingxiang;Jiang Yu;Shi Haibin;Liu Weiai(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China;The Second Affiliated Hospital of Hainan Medical University,Haikou 570216,China;Zhangjiajie Hospital of Traditional Chinese Medicine,Zhangjiajie 427000,China)

机构地区：[1]湖南中医药大学第二附属医院,长沙410005 [2]海南医学院第二附属医院,海口570216 [3]张家界市中医院,张家界427000

出　　处：《世界科学技术-中医药现代化》2023年第5期1673-1680,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖南省自然科学基金委员会面上项目(2020JJ4472):基于心胃相关理论从HPA轴探讨电针内关、公孙穴对FD大鼠胃肠动力的调节机制,负责人:刘未艾;湖南省中医药管理局湖南省中医药科研计划重点项目(2021026):基于心胃相关理论从CRF1-CRF2受体信号通路探究电针内关、公孙穴对FD大鼠内脏高敏性的调控机制,负责人:刘未艾;湖南中医药大学研究生科研创新课题(2020CX47):基于心胃相关理论探讨电针内关、公孙穴对FD大鼠杏仁核、海马兴奋-抑制的调控机制,负责人:覃思敏

摘　　要：目的观察电针内关、公孙穴对FD大鼠海马GluR1、GluR2及内脏敏感性的影响,探讨其可能的作用机制及腧穴的特异性,为临床运用提供一定的实验依据。方法将50只大鼠随机分为空白组、模型组、双穴组、非经非穴组和西药组5组,每组各10只;空白组正常饲养,模型组采用复合病因法造模,双穴组造模后行电针内关、公孙穴治疗,非经非穴组造模后行电针非经非穴治疗,西药组造模后灌服黛力新+莫沙比利治疗,各组每日治疗1次,共21天;治疗后采用结直肠扩张观察大鼠的内脏敏感性,HE染色观察海马神经元结构,Western Blot及IHC观察海马内GluR1、GluR2变化。结果与空白组相比,模型组大鼠内脏痛阈值明显降低,海马GluR1含量升高、GluR2蛋白含量下降(P<0.01);与模型组相比,双穴组和西药组内脏痛阈值明显增高,海马GluR1蛋白含量下调、GluR2蛋白含量增加(P<0.01,P<0.05),非经非穴组无差异(P>0.05);与非经非穴组对比,双穴组大鼠内脏痛阈值显著升高,显著降低海马GluR1、上调GluR2蛋白含量(P<0.01);与西药组对比,两组无显著性差异(P>0.05)。HE染色结果表明,空白组海马神经元细胞结构清晰,形态正常;与空白组相比,模型组海马可见个别神经元细胞变性,固缩,细胞间隙变大,胞体增大,空泡样性状明显;与模型组相比,双穴组及西药组海马神经元细胞结构较为清晰,趋于完整,细胞间隙变小,未见固化萎缩的神经元及空泡样性状改变;与非经非穴组相比,双穴组海马神经元细胞结构更为清晰,细胞间隙较小。结论电针内关、公孙穴能有效改善FD大鼠内脏敏感性,疗效较非经非穴更优,可能与存在腧穴特异性相关。其可下调大鼠海马内GluR1、上调GluR2蛋白含量,改善海马神经元细胞结构,这可能是其治疗FD的作用机制之一。Objective To observe the effects of electroacupuncture Sp4 and pC6 points on hippocampal GluR1 and GluR2 receptors and visceral sensitivity of FD rats,and to explore the possible mechanism and specificity of the points,so as to provide experimental basis for clinical application.Methods 50 rats were randomly divided into Blank group(BG).There are 10 rats in each group:Modle Group(MG),Drug group(DG),Electroacupuncture Sp4 and pC6 group(EAG),and Non-meridian and non-acupoint points(NNAG),with 10 rats in each group.The BG was fed normally,the MG was modeled by compound etiology method,the EAG was treated with electroacupuncture Sp4 and pC6 points after modeling,the NNAG was treated with electroacupuncture Sp4 and pC6 points after modeling,and the DG was treated with dailixin combination mosabili after modeling,once a day for 21 days.Visceral sensitivity was observed by abdominal wall withdrawal reflex after treatment,hippocampal neuron structure was observed by HE staining,GluR1 and GluR2 changes in hippocampus were observed by Western Blot and IHC.Results Compared with BG,the visceral pain threshold of MG was significantly lower,and the hippocampal GluR1 content was increased and GluR2 protein content was decreased(P<0.01).Compared with MG,the visceral pain threshold was significantly higher in the EAG and DG,and the hippocampal GluR1 protein content was down-regulated and GluR2 protein content was increased(P<0.01,P<0.05),but there was no difference in the NNAG(P>0.05).Compared with the NNAG,the visceral pain threshold of rats in the EAG was significantly increased,and GluR1 and GluR2 protein contents in hippocampus were significantly decreased(P<0.01).Compared with western DG,there was no significant difference between the two groups(P>0.05).HE staining results showed that the structure of hippocampal neurons in BG was clear and normal.Compared with the BG,some neurons in the MG were found to be denaturated,pyknosis,cell space enlarged,cell body enlarged and vacuolated.Compared with the MG,the structure of hippoc

关 键 词：电针 内关穴 公孙穴 功能性消化不良 GLUR1 GLUR2 

分 类 号：R224.2[医药卫生—中医基础理论]